关键信息
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基因名
EEF1D
- 应用
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别名
EEF1D；EF1D;Elongation factor 1-delta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P29692
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表达区间
1-281aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMATNFLAHEKIWFDKFKYDDAERRFYEQMN GPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGEL VVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQ HVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLR QYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGA SKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNK I
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分子量
33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EEF1D, or eukaryotic translation elongation factor 1 delta, is a crucial protein involved in the translation process of eukaryotic cells, playing a pivotal role in the elongation phase of mRNA translation. Research has indicated that EEF1D is not only essential for protein synthesis but is also linked to several cellular processes, including cell proliferation, apoptosis, and stress responses. Its involvement in the regulation of the translation machinery makes it a point of interest in various pathological conditions, including cancer, where dysregulation of protein synthesis is common. Recent studies have suggested that EEF1D may have additional roles beyond its canonical function, contributing to cellular signaling pathways and possibly influencing the tumor microenvironment. The recombinant expression of EEF1D has been pursued to better understand its structure-function relationships and interactions with other cellular components. This research seeks to elucidate the potential of EEF1D as a therapeutic target, providing insights into its mechanistic roles and possibilities for intervention in diseases characterized by altered protein synthesis. Understanding the dynamics of EEF1D through recombinant protein studies could pave the way for novel strategies in drug development and therapeutic applications, highlighting its relevance in both fundamental biology and clinical settings.
