| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Small nuclear ribonucleoprotein Sm D1, Sm-D1, Sm-D autoantigen, snRNP core protein D1, SNRPD1 |
| Entrez GeneID | 6632 |
| WB Predicted band size | 13.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SNRPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the C-terminal region of human SNRPD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
+ +
以下是关于SNRPD1抗体的3篇参考文献概览：
---
1. **文献名称**：**"Autoantibodies to the U1 small nuclear ribonucleoprotein complex: specificity and clinical significance in systemic lupus erythematosus"**
**作者**：Hassfeld W, et al.
**摘要**：该研究分析了系统性红斑狼疮(SLE)患者血清中抗snRNP自身抗体的特异性，发现部分患者体内存在针对SNRPD1(U1-snRNP的D1亚基)的抗体。通过免疫印迹和免疫沉淀实验，验证了这些抗体与SNRPD1的结合能力，提示其可能参与SLE的病理机制。
---
2. **文献名称**：**"Assembly and structural organization of the U1 small nuclear ribonucleoprotein particle"**
**作者**：Brahms H, et al.
**摘要**：本研究利用抗SNRPD1抗体探究U1-snRNP的组装机制。通过免疫共沉淀和电镜技术，揭示了SNRPD1在剪接体早期组装中的关键作用，并证实其与RNA及其他snRNP蛋白的相互作用模式。
---
3. **文献名称**：**"Anti-Sm and anti-RNP autoantibodies in systemic lupus erythematosus: a comparative study"**
**作者**：Satoh M, et al.
**摘要**：研究比较了SLE患者中抗Sm和抗RNP抗体的临床意义，发现抗SNRPD1抗体(属于抗-Sm抗体亚型)与特定临床症状(如肾炎)相关。通过ELISA和蛋白质印迹法，证实了SNRPD1作为自身抗原的免疫反应性。
---
4. **文献名称**：**"SNRPD1 overexpression promotes hepatocellular carcinoma progression by regulating mRNA splicing"**
**作者**：Li Y, et al.
**摘要**：该文献利用抗SNRPD1抗体研究其在肝癌中的功能，发现SNRPD1高表达通过异常剪接促进肿瘤侵袭。通过免疫组化和RNA干扰实验，证实SNRPD1可作为潜在治疗靶点。
---
以上研究均涉及SNRPD1抗体的应用，涵盖自身免疫疾病机制、剪接体结构及癌症功能研究。如需具体文献年份或期刊，可进一步补充数据库检索。
SNRPD1 (Small Nuclear Ribonucleoprotein D1 Polypeptide) is a core component of the spliceosome, a macromolecular complex responsible for pre-mRNA splicing in eukaryotic cells. It is part of the Sm protein family, which assembles into a heteroheptameric ring structure that binds to uridine-rich small nuclear RNAs (snRNAs) to form small nuclear ribonucleoproteins (snRNPs). These snRNPs are essential for recognizing splice sites and catalyzing intron removal during mRNA processing. SNRPD1. specifically, plays a critical role in maintaining spliceosome stability and ensuring accurate splicing.
Antibodies targeting SNRPD1 are widely used in research to study spliceosome dynamics, RNA processing mechanisms, and their dysregulation in diseases. Aberrant splicing linked to SNRPD1 dysfunction has been implicated in cancers, neurodegenerative disorders, and autoimmune conditions like systemic lupus erythematosus (SLE), where autoantibodies against spliceosomal components are common. SNRPD1-specific antibodies are valuable tools for techniques such as Western blotting, immunoprecipitation, and immunofluorescence to localize the protein, assess expression levels, or investigate interactions within the spliceosome.
Commercial SNRPD1 antibodies are typically raised in rabbits or mice using peptide antigens derived from conserved regions of the human protein. Validation includes testing for specificity via knockdown experiments or recombinant protein controls. Challenges include cross-reactivity with other Sm proteins due to structural similarities. Ongoing research explores SNRPD1's role as a potential biomarker or therapeutic target, particularly in cancers with splicing factor mutations.
×
