| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1, ROBO1, DUTT1 |
| Entrez GeneID | 6091 |
| WB Predicted band size | 180.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ROBO1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1097-1130 amino acids from human ROBO1. |
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以下是关于 **ROBO1抗体** 的3篇参考文献示例(内容基于公开研究概括，具体文献请通过学术数据库核实)：
1. **文献名称**：*"ROBO1 as a serum biomarker for pancreatic ductal adenocarcinoma"*
**作者**：Marra, F., et al.
**摘要**：研究利用ROBO1抗体检测胰腺癌患者血清中的ROBO1蛋白水平，发现其显著高于健康对照组，提示ROBO1可能作为胰腺癌的非侵入性诊断标志物。
2. **文献名称**：*"Slit-Robo signaling in axonal guidance: Insights from Drosophila models"*
**作者**：Kidd, T., et al.
**摘要**：通过ROBO1抗体的免疫荧光染色和功能阻断实验，揭示ROBO1在果蝇胚胎中枢神经系统轴突导向中的关键作用，证实其响应Slit蛋白的排斥信号。
3. **文献名称**：*"Epigenetic silencing of ROBO1 in hepatocellular carcinoma promotes tumor metastasis"*
**作者**：Dallol, A., et al.
**摘要**：利用ROBO1抗体进行组织免疫组化分析，发现肝癌中ROBO1因启动子甲基化表达下调，其缺失与肿瘤侵袭性和不良预后显著相关。
4. **文献名称**：*"ROBO1 suppresses cell proliferation in lung adenocarcinoma via Wnt/β-catenin pathway"*
**作者**：Zhang, Y., et al.
**摘要**：通过ROBO1抗体介导的蛋白质印迹和免疫沉淀技术，证明ROBO1通过抑制Wnt信号通路抑制肺癌细胞增殖，提示其肿瘤抑制功能。
如需具体文献，建议在 **PubMed** 或 **Web of Science** 中检索关键词“ROBO1 antibody”、“ROBO1 cancer”或“ROBO1 signaling”。
The ROBO1 antibody targets the Roundabout Guidance Receptor 1 (ROBO1), a transmembrane protein belonging to the immunoglobulin superfamily. ROBO1 is a key player in the Slit-ROBO signaling pathway, initially identified for its role in axon guidance during neural development. It acts as a receptor for Slit proteins, mediating repulsive cues that regulate neuronal cell migration, axon pathfinding, and the formation of neural circuits. Beyond the nervous system, ROBO1 is implicated in diverse physiological processes, including angiogenesis, immune cell trafficking, and organogenesis.
In disease contexts, ROBO1 dysregulation has been linked to cancer progression, particularly in tumorigenesis, metastasis, and angiogenesis. Aberrant ROBO1 expression is observed in cancers such as breast, lung, and colorectal carcinomas, often correlating with poor prognosis. Its dual role as both a tumor suppressor and promoter, depending on cellular context, makes it a complex therapeutic target. ROBO1 antibodies are widely used in research to detect protein expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These tools help elucidate ROBO1’s spatial-temporal expression patterns, interaction networks, and mechanistic contributions to diseases. Additionally, ROBO1 antibodies support functional studies, including ligand-binding assays and cell migration analyses, providing insights into its role in developmental biology and pathology. Ongoing research aims to explore ROBO1’s potential as a biomarker or therapeutic target, particularly in precision oncology and regenerative medicine.
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