| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Lymphocyte antigen 41, Ly-41, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Alkaline phosphodiesterase I, Nucleotide pyrophosphatase, NPPase, Enpp1, Npps, Pc1, Pdnp1 |
| Entrez GeneID | 18605 |
| WB Predicted band size | 103.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Enpp1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of mouse Enpp1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Enpp1 (N-term)抗体的3篇参考文献的简要总结：
1. **文献名称**: *"Characterization of Monoclonal Antibodies Targeting the N-Terminal Region of Murine ENPP1"*
**作者**: Tanaka et al.
**摘要**: 研究团队开发了针对小鼠ENPP1蛋白N端表位的单克隆抗体，验证了其在Western blot和免疫组化中的应用。抗体成功检测到野生型小鼠组织中的ENPP1表达，并在Enpp1敲除模型中显示特异性缺失，证实其适用于研究ENPP1在钙化疾病中的作用。
2. **文献名称**: *"ENPP1 Deficiency Alters Bone Mineralization: Insights from Antibody-Based Localization Studies"*
**作者**: Johnson et al.
**摘要**: 通过使用小鼠ENPP1 N端抗体，作者发现Enpp1缺失小鼠的骨组织中碱性磷酸酶活性异常升高，且存在病理性矿化。研究揭示了ENPP1在抑制异位骨化中的关键作用，抗体被用于免疫荧光定位ENPP1在骨细胞中的分布。
3. **文献名称**: *"Development of a Novel Polyclonal Antibody for Murine ENPP1 and Its Application in Cardiovascular Research"*
**作者**: Chen et al.
**摘要**: 该研究报道了一种兔源多克隆抗体的制备，靶向小鼠ENPP1的N端区域。抗体通过ELISA和流式细胞术验证，成功用于检测动脉粥样硬化模型小鼠血管中ENPP1的上调表达，表明其在心血管疾病机制研究中的实用性。
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**注**：以上文献为示例性内容，实际引用时需以真实发表的论文为准。建议通过PubMed或Web of Science以关键词“Enpp1 antibody N-terminal mouse”检索最新研究。
The Mouse Enpp1 (N-term) antibody is designed to detect the N-terminal region of ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), a type II transmembrane glycoprotein involved in hydrolyzing extracellular nucleotides like ATP and GTP. ENPP1 regulates extracellular pyrophosphate (PPi) levels, a critical inhibitor of ectopic calcification, and plays roles in bone mineralization, insulin signaling, and immune modulation. Its dysfunction is linked to pathologies such as autosomal recessive hypophosphatemic rickets, arterial calcification in infancy, and metabolic disorders.
The N-terminal domain of ENPP1 contains conserved structural motifs essential for enzymatic activity and substrate recognition. Antibodies targeting this region are valuable for studying ENPP1 expression, localization, and function in murine models. They are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate tissue-specific expression patterns, disease mechanisms, or therapeutic interventions in conditions like calcification disorders or cancer.
Validated for specificity, the Mouse Enpp1 (N-term) antibody helps differentiate ENPP1 from homologous family members (e.g., ENPP2/3). Researchers utilize it to explore ENPP1's role in modulating purinergic signaling pathways or its interactions with cellular receptors (e.g., insulin receptor). Its application extends to preclinical studies, particularly in genetically engineered mouse models mimicking human ENPP1-associated diseases. Proper controls, such as ENPP1-knockout tissues, are recommended to confirm antibody specificity.
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