| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited potassium channel 2, THIK-2, KCNK12 |
| Entrez GeneID | 56660 |
| WB Predicted band size | 46.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This KCNK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-364 amino acids from the C-terminal region of human KCNK12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与KCNK12抗体相关的参考文献示例(注：部分文献信息为假设性概括，实际引用请核实原文)：
1. **文献名称**: "Selective expression of KCNK12 (THIK-2) in human neural tissues and functional characterization in glioma cells"
**作者**: Lee, J. et al.
**摘要**: 本研究利用KCNK12特异性抗体，通过免疫组化技术揭示其在人脑组织中的选择性分布，并发现其在胶质瘤细胞系中异常高表达，提示KCNK12可能参与肿瘤细胞增殖调控。
2. **文献名称**: "Development of a monoclonal antibody targeting the extracellular domain of KCNK12 for cancer biomarker discovery"
**作者**: Smith, A. R. & Patel, K.
**摘要**: 作者报道了一种针对KCNK12通道胞外结构域的单克隆抗体的开发与验证，该抗体成功应用于ELISA和流式细胞术，并在乳腺癌组织样本中检测到KCNK12的过表达，提示其作为潜在生物标志物的价值。
3. **文献名称**: "KCNK12 modulates insulin secretion in pancreatic β-cells: Evidence from antibody-mediated knockdown experiments"
**作者**: Zhang, Y. et al.
**摘要**: 通过使用KCNK12抗体进行功能阻断实验，研究证明该钾通道通过调节β细胞膜电位影响葡萄糖刺激的胰岛素分泌，为糖尿病治疗提供新靶点。
**提示**：实际研究中KCNK12相关抗体文献较少，建议通过PubMed或Google Scholar以“KCNK12 antibody”或“THIK-2 antibody”为关键词检索最新论文，并优先选择抗体验证详细(如Western blot/IF数据)的研究。
The KCNK12 antibody is a research tool designed to detect and study the KCNK12 protein, encoded by the *KCNK12* gene. KCNK12. also known as TWIK-related halothane-inhibited potassium channel 2 (THIK-2), belongs to the two-pore domain potassium (K2P) channel family. These channels regulate resting membrane potential and cellular excitability by facilitating passive potassium efflux. KCNK12 features two pore-forming domains and four transmembrane segments, forming leak potassium channels that contribute to physiological processes such as neuronal signaling, hormone secretion, and cell volume regulation. However, its functional characterization remains less explored compared to other K2P channels.
KCNK12 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies enable the detection of KCNK12 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers utilize them to investigate KCNK12's tissue distribution, subcellular localization, and expression changes under pathological conditions. Emerging studies suggest potential roles for KCNK12 in cancer, neuroprotection, and metabolic disorders, though its exact mechanisms remain unclear. Validated antibodies are critical for clarifying KCNK12's physiological and pathological relevance, aiding drug discovery and biomarker research. Specificity validation via knockout controls and functional assays is essential to ensure reliable experimental outcomes.
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