| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, bHLHb21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, TCF-3, Transcription factor ITF-1, TCF3, BHLHB21, E2A, ITF1 |
| Entrez GeneID | 6929 |
| WB Predicted band size | 67.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TCF3抗体的3篇代表性文献的概括(注：文献为示例性描述，实际引用时请核实具体来源)：
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1. **文献名称**：*TCF3 (E2A) regulates B-cell development via chromatin remodeling in mice*
**作者**：Murre, C., et al.
**摘要**：本研究利用TCF3特异性抗体通过ChIP-seq技术，揭示了TCF3在B细胞分化过程中通过调控染色质可及性，激活关键基因(如EBF1和PAX5)表达的分子机制，证实了其在早期B细胞发育中的必要性。
2. **文献名称**：*Antibody-based detection of TCF3 fusion proteins in pediatric acute lymphoblastic leukemia*
**作者**：Roberts, K.G., et al.
**摘要**：通过Western blot和免疫荧光技术，研究者使用TCF3抗体在儿童急性淋巴细胞白血病样本中检测到TCF3-PBX1融合蛋白，为临床诊断和靶向治疗提供了分子标志物依据。
3. **文献名称**：*TCF3-mediated transcriptional networks in pluripotent stem cell maintenance*
**作者**：Hnisz, D., et al.
**摘要**：研究通过TCF3抗体的免疫共沉淀(Co-IP)实验，揭示了TCF3与OCT4/SOX2复合物的相互作用，阐明了其如何在多能干细胞中协调自我更新与分化相关基因的转录平衡。
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**说明**：以上文献为示例性概括，实际研究中请查阅具体数据库(如PubMed)获取真实文献。若需精确引用，建议使用关键词“TCF3 antibody”、“E2A immune localization”等检索近年研究。
The TCF3 antibody targets the transcription factor 3 (TCF3), also known as E2A, a protein encoded by the *TCF3* gene in humans. TCF3 belongs to the E-protein family of basic helix-loop-helix (bHLH) transcription factors, which regulate gene expression by binding to specific DNA sequences. It plays critical roles in embryonic development, cell differentiation, and immune system regulation. In particular, TCF3 is essential for B-cell and T-cell maturation, influencing lymphocyte development by controlling the expression of genes involved in lineage commitment and immunoglobulin rearrangement.
TCF3 antibodies are widely used in research to study its expression, localization, and function in normal and pathological contexts. For example, aberrant TCF3 activity is linked to cancers, including acute lymphoblastic leukemia (ALL), where chromosomal translocations (e.g., *TCF3-PBX1*) create oncogenic fusion proteins. Researchers employ these antibodies in techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to investigate TCF3's role in transcriptional networks and disease mechanisms.
Additionally, TCF3 is implicated in maintaining pluripotency in embryonic stem cells by repressing differentiation genes. Its dual role in promoting lineage-specific differentiation and sustaining stemness highlights its regulatory complexity. TCF3 antibodies thus serve as vital tools for dissecting developmental biology, immunology, and oncogenesis pathways.
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