| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial, CybS, CII-4, QPs3, Succinate dehydrogenase complex subunit D, Succinate-ubiquinone oxidoreductase cytochrome b small subunit, Succinate-ubiquinone reductase membrane anchor subunit, SDHD, SDH4 |
| Entrez GeneID | 6392 |
| WB Predicted band size | 17.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SDHD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SDHD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SDHD(N-term)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Germline SDHD mutations in familial phaeochromocytoma and paraganglioma"*
**作者**: Baysal BE, et al.
**摘要**: 该研究通过Western blot和免疫组化分析，使用SDHD N端特异性抗体，证实SDHD基因种系突变导致家族性副神经节瘤患者中SDHD蛋白表达缺失，揭示了突变与肿瘤发生的关联。
2. **文献名称**: *"Immunohistochemical localization of SDHD in human tissues: implications for SDH deficiency syndromes"*
**作者**: Hensen EF, et al.
**摘要**: 研究利用SDHD(N-term)抗体进行组织染色，系统描述了SDHD蛋白在正常和肿瘤组织中的分布模式，为琥珀酸脱氢酶(SDH)缺陷相关疾病的诊断提供了形态学依据。
3. **文献名称**: *"Structural and functional consequences of SDHD mutations in mitochondrial complex II"*
**作者**: Richter S, et al.
**摘要**: 通过免疫沉淀和蛋白质互作实验，结合N端抗体检测SDHD的稳定性，发现特定突变导致复合体II组装异常，影响线粒体呼吸功能，提示突变机制与蛋白降解相关。
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**注**：以上文献为示例，实际检索需通过PubMed或Google Scholar等平台输入关键词(如“SDHD antibody N-terminal”)获取具体信息。部分研究可能涉及商业抗体(如Abcam #ab123或CST #4567)，可参考供应商的产品引用文献列表。
The SDHD (N-term) antibody targets the N-terminal region of the succinate dehydrogenase complex subunit D (SDHD), a key component of mitochondrial complex II (succinate-ubiquinone oxidoreductase) in the electron transport chain. SDHD, encoded by the SDHD gene on chromosome 11q23. anchors the complex to the mitochondrial inner membrane and is critical for converting succinate to fumarate in the Krebs cycle while transferring electrons to ubiquinone. Mutations in SDHD are linked to hereditary paraganglioma-pheochromocytoma syndrome (PGL1), a genetic disorder predisposing individuals to neuroendocrine tumors, particularly in the head, neck, and adrenal glands. SDHD acts as a tumor suppressor, and its loss disrupts cellular energy metabolism, potentially driving tumorigenesis through pseudohypoxic signaling.
The SDHD (N-term) antibody is widely used in research to study SDHD expression, localization, and function via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It helps identify SDHD deficiencies in tumor samples, aiding in molecular diagnostics and stratification of patients with SDHD-related syndromes. As SDHD mutations are inherited in an autosomal dominant manner with maternal imprinting, this antibody also supports genetic and functional studies exploring genotype-phenotype correlations. Its specificity for the N-terminal region ensures detection of full-length SDHD, distinguishing it from potential truncation variants linked to disease. This tool is essential for advancing understanding of mitochondrial biology and cancer metabolism.
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