| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1 |
| Entrez GeneID | 79727 |
| WB Predicted band size | 22.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the Central region of human LIN28A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于LIN28A抗体的3篇参考文献(名称、作者及摘要概括)：
1. **文献名称**：*Selective blockade of microRNA processing by Lin28*
**作者**：Viswanathan, S.R. et al.
**摘要**：研究揭示了LIN28A通过结合let-7 microRNA前体抑制其成熟的过程，实验中使用LIN28A抗体进行免疫沉淀(RIP)和Western blot验证其与RNA的相互作用。
2. **文献名称**：*Lin28 promotes transformation and is associated with advanced human malignancies*
**作者**：Peng, S. et al.
**摘要**：通过免疫组化(IHC)和Western blot分析，证明LIN28A在多种癌症(如结直肠癌、卵巢癌)中高表达，并与患者预后不良相关，研究依赖LIN28A抗体检测蛋白表达水平。
3. **文献名称**：*LIN28A regulates stem cell metabolism and pluripotency via mitochondrial function*
**作者**：Zhang, J. et al.
**摘要**：探讨LIN28A通过调控线粒体代谢维持多能性，实验中利用LIN28A抗体进行免疫荧光染色和ChIP-seq，证实其在胚胎干细胞中的关键作用。
4. **文献名称**：*Lin28a activates cytoplasmic patterning in mouse embryos*
**作者**：West, J.A. et al.
**摘要**：研究LIN28A在早期胚胎发育中的功能，通过LIN28A抗体的免疫共沉淀和亚细胞定位分析，揭示其调控翻译过程的机制。
(注：以上文献信息为示例，实际引用时需核对原文准确性。)
LIN28A is an RNA-binding protein that plays a critical role in embryonic development, stem cell pluripotency, and cellular reprogramming. It functions by binding to specific RNA molecules, such as let-7 microRNAs, to regulate their maturation and post-transcriptional expression. LIN28A is highly expressed during early embryogenesis but becomes restricted to specific tissues in adulthood. Its dysregulation is linked to various cancers, where overexpression correlates with poor prognosis and aggressive tumor behavior.
Antibodies targeting LIN28A are essential tools for studying its expression patterns, molecular interactions, and functional roles in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect LIN28A protein levels in tissues, stem cells, or cancer samples. Researchers also employ LIN28A antibodies to investigate mechanisms underlying pluripotency maintenance, tissue regeneration, and oncogenesis.
Given LIN28A's association with cancer progression and its potential as a therapeutic target, specific and validated antibodies are crucial for diagnostic and research applications. However, challenges remain in ensuring antibody specificity due to homology with its paralog, LIN28B. Recent studies emphasize the need for rigorous validation using knockout controls to minimize off-target signals, enhancing reliability in experimental outcomes.
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