| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Fizzy-related protein homolog, Fzr, CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1, FZR1, CDH1, FYR, FZR, KIAA1242 |
| Entrez GeneID | 51343 |
| WB Predicted band size | 55.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This FZR antibody is generated from a mouse immunized with a recombinant protein between1-496 amino acids from human FZR. |
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以下是关于FZR(Fizzy-related，也称CDC20同源物)抗体的研究文献示例(文献为模拟示例，供参考)：
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1. **文献名称**：*FZR1/CDH1-mediated regulation of the anaphase-promoting complex in neuronal development*
**作者**：Marín, I., et al.
**摘要**：研究FZR1(CDH1)通过APC/C复合体调控神经元细胞周期退出和分化的机制，利用FZR抗体进行免疫沉淀和Western blot分析，证实其在神经元分化中的关键作用。
2. **文献名称**：*Role of FZR1 in maintaining genomic stability through APC/C-dependent proteolysis*
**作者**：Garcia-Higuera, I., et al.
**摘要**：探讨FZR1通过调控APC/C依赖的蛋白质降解来维持基因组稳定性，研究中使用特异性FZR抗体验证其在DNA损伤修复中的表达变化及功能缺失表型。
3. **文献名称**：*FZR1 antibody-based detection of APC/C activity in cancer cell lines*
**作者**：Zhang, J., et al.
**摘要**：开发一种基于FZR抗体的实验方法，用于定量分析多种癌细胞系中APC/C活性，揭示FZR1表达水平与肿瘤细胞增殖及化疗敏感性的相关性。
4. **文献名称**：*Drosophila Fizzy-related controls mitotic exit by targeting Cyclin B for degradation*
**作者**：Sigrist, S.J., Lehner, C.F.
**摘要**：通过果蝇模型研究FZR在调控有丝分裂退出中的作用，利用FZR抗体进行免疫荧光染色，证明其通过降解Cyclin B促进细胞周期转换。
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**注**：以上文献为示例，实际引用时请以真实发表的论文为准。建议通过PubMed或Google Scholar搜索关键词“FZR antibody”、“CDC20 homolog”或“APC/C CDH1”获取相关研究。
FZR (Fizzy-related), also known as CDH1 (Cdc20 Homolog 1), is a regulatory subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase critical for cell cycle progression. Discovered in the late 1990s, FZR shares homology with Cdc20. another APC/C coactivator, but exhibits distinct functional roles. While Cdc20 primarily drives APC/C activity during metaphase-to-anaphase transition, FZR is essential for maintaining APC/C function in late mitosis and G1 phase, facilitating cyclin degradation, mitotic exit, and cell cycle arrest. FZR also regulates non-cell cycle processes, including metabolism, neuronal development, and genome stability.
FZR antibodies are vital tools for studying these mechanisms. They enable detection of FZR expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Researchers employ FZR antibodies to explore its role in development, differentiation, and diseases. For instance, FZR dysregulation is linked to cancer, neurodegeneration, and infertility. Knockout studies in model organisms (e.g., mice, Drosophila) highlight its necessity in embryogenesis and tissue homeostasis.
Commercial FZR antibodies are typically raised against conserved epitopes, with validation in specific applications. However, cross-reactivity with Cdc20 or homologs requires careful experimental design. Ongoing research continues to unravel FZR's context-dependent regulation and therapeutic potential.
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