| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lipopolysaccharide-induced tumor necrosis factor-alpha factor, LPS-induced TNF-alpha factor, Small integral membrane protein of lysosome/late endosome, p53-induced gene 7 protein, LITAF, PIG7, SIMPLE |
| Entrez GeneID | 9516 |
| WB Predicted band size | 17.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This LITAF antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 31-60 amino acids from the human LITAF. |
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以下是关于LITAF (N-Term)抗体的参考文献示例：
1. **"Molecular cloning and characterization of a novel LPS-induced TNF-α factor (LITAF) from human monocytes"**
- **作者**: Myokai, F., et al.
- **摘要**: 该研究首次克隆并鉴定了人单核细胞中的LITAF蛋白，开发了针对其N端的多克隆抗体。通过Western blot和Northern blot分析，证实LITAF在LPS刺激后表达上调，并参与炎症反应中的TNF-α调控。
2. **"Mutations in the LITAF/SIMPLE gene in Charcot-Marie-Tooth disease type 1C"**
- **作者**: Street, V.A., et al.
- **摘要**: 文章发现LITAF基因突变与CMT1C型周围神经病变相关。利用LITAF (N-Term)抗体进行免疫组化分析，揭示了突变蛋白在施万细胞中的异常定位，提示其在髓鞘维持中的作用。
3. **"SIMPLE interacts with LITAF to regulate intracellular protein trafficking"**
- **作者**: Shirk, A.J., et al.
- **摘要**: 研究通过共免疫沉淀和免疫荧光技术(使用N端抗体)，证实LITAF与SIMPLE蛋白在溶酶体膜上的相互作用，为二者在细胞内运输和疾病中的功能联系提供证据。
4. **"LITAF modulates autophagy and apoptosis in colorectal cancer via the mTOR pathway"**
- **作者**: Zhang, Y., et al.
- **摘要**: 该文献利用LITAF (N-Term)抗体进行Western blot和免疫荧光实验，发现LITAF在结直肠癌细胞中通过调控mTOR通路影响自噬和凋亡，为癌症治疗提供潜在靶点。
注：以上文献信息为示例性质，实际引用时需核对原文准确性。
The LITAF (N-Term) antibody targets the N-terminal region of the LITAF protein (Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Factor), also known as SIMPLE (Small Integral Membrane Protein of the Lysosome/Late Endosome). LITAF is a ubiquitously expressed protein involved in intracellular protein degradation pathways, particularly in endosomal sorting and lysosomal trafficking. It functions as a regulator of TNF-α and other cytokines by modulating their transcription and secretion, linking it to inflammatory responses and immune regulation.
Structurally, LITAF contains an N-terminal early endosomal targeting domain and a C-terminal lysosomal sorting motif. The N-terminal region is critical for its subcellular localization and interaction with proteins like CHMP4B, which are essential for the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. Mutations in the LITAF gene are associated with Charcot-Marie-Tooth disease type 1C (CMT1C), a hereditary peripheral neuropathy, highlighting its role in maintaining peripheral nerve integrity.
The LITAF (N-Term) antibody is widely used in research to study protein trafficking, cytokine regulation, and neurodegenerative mechanisms. It aids in detecting LITAF expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Its specificity for the N-terminal epitope makes it valuable for distinguishing full-length LITAF from potential cleavage products or isoforms. Studies leveraging this antibody have advanced understanding of LITAF's dual roles in immune signaling and cellular proteostasis, as well as its implications in cancer, inflammation, and neuropathies.
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