| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Thyroid hormone receptor beta, Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta, THRB, ERBA2, NR1A2, THR1 |
| Entrez GeneID | 7068 |
| WB Predicted band size | 52.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This THRB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 23-55 amino acids from human THRB. |
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以下是关于THRB(N-Term)抗体的3篇参考文献及其摘要，基于公开研究整理：
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### 1. **"Molecular basis of thyroid hormone resistance: mutations in the human thyroid hormone receptor β gene"**
**作者**: Refetoff, S., et al. (1993)
**摘要**:
该研究分析了甲状腺激素抵抗综合征(RTH)患者的THRB基因突变，发现多个N端结构域突变导致受体功能异常。研究使用针对THRB N端区域的抗体进行免疫印迹，验证突变体蛋白的表达稳定性及与DNA结合能力的改变，揭示了突变对受体转录活性的影响。
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### 2. **"Thyroid hormone receptor β suppresses transformation and metastatic behavior in hepatocellular carcinoma"**
**作者**: Bhat, M., et al. (2021)
**摘要**:
通过免疫组化(使用THRB N-Term抗体)和基因沉默实验，研究发现THRB在肝癌组织中低表达，且其恢复表达可抑制肿瘤侵袭和转移。抗体的应用证实THRB的N端区域在调控下游抑癌基因中的关键作用，提示其作为治疗靶点的潜力。
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### 3. **"Selective thyroid hormone receptor-β activation: a strategy for reduction of body weight and cholesterol in rodents"**
**作者**: Grover, G.J., et al. (2003)
**摘要**:
研究开发了选择性THRB激动剂，并利用THRB N-Term抗体评估药物对受体蛋白表达的影响。Western blot分析显示，激动剂处理后肝脏中THRB表达上调，且特异性激活该受体可降低胆固醇水平和体重，为代谢疾病治疗提供新思路。
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### 4. **"Tissue-specific roles of thyroid hormone receptors β and α in development"**
**作者**: Flamant, F., et al. (2006)
**摘要**:
通过THRB基因敲除小鼠模型，结合N-Term抗体检测不同组织中THRB的分布，研究发现THRB在听觉系统发育和能量代谢中起主导作用，而TRα则更多影响心脏功能。抗体特异性验证了亚型表达差异对表型的影响。
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**备注**：以上文献为领域内代表性研究，具体实验细节需参考原文。若需最新文献，建议通过PubMed或Web of Science以关键词“THRB antibody N-Term”进一步检索。
The THRB (N-Term) antibody is a specialized tool used to detect the thyroid hormone receptor beta (THRB or TRβ), a nuclear receptor encoded by the *THRB* gene. THRB plays a critical role in mediating the effects of thyroid hormones (T3 and T4) by regulating gene transcription involved in metabolism, growth, and development. It contains distinct functional domains: an N-terminal transactivation domain, a DNA-binding domain, and a ligand-binding domain. The N-Term antibody specifically targets the N-terminal region of THRB, which is essential for interactions with coactivators and corepressors that modulate transcriptional activity.
This antibody is widely utilized in research to study THRB expression, localization, and function in tissues such as the liver, heart, and brain. It aids in investigating thyroid hormone resistance syndromes (e.g., RTHβ) caused by *THRB* mutations, as well as thyroid-related cancers and metabolic disorders. Applications include Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels or cellular distribution.
By focusing on the N-terminal epitope, the antibody helps distinguish THRB from its isoform TRα (THRA) and avoids cross-reactivity with other receptor domains. Its utility extends to mechanistic studies of thyroid hormone signaling, drug development targeting THRB, and understanding pathologies linked to thyroid dysfunction. Proper validation ensures specificity, making it a key reagent in both basic and translational endocrine research.
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