| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tyrosine-protein phosphatase non-receptor type 12, 3.1.3.48, PTP-PEST, Protein-tyrosine phosphatase G1, PTPG1, PTPN12 |
| Entrez GeneID | 5782 |
| WB Predicted band size | 88.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PTPN12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 724-756 amino acids from human PTPN12. |
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以下是3-4条关于PTPN12抗体的参考文献及其摘要概括：
1. **"PTPN12 inhibits breast cancer progression by regulating HER2 signaling"**
- **作者**: Tao Wang et al.
- **摘要**: 该研究揭示PTPN12作为肿瘤抑制因子，通过去磷酸化HER2受体酪氨酸激酶，抑制下游MAPK和PI3K/AKT信号通路，从而阻碍HER2阳性乳腺癌的增殖和转移。
2. **"PTPN12 suppresses hepatocellular carcinoma by modulating YAP oncogenic activity"**
- **作者**: Xin-Hai Zheng et al.
- **摘要**: 研究发现PTPN12通过去磷酸化转录共激活因子YAP，抑制其核定位和促癌功能，在肝癌中发挥抑癌作用，并提示PTPN12缺失与患者预后不良相关。
3. **"A genome-wide CRISPR screen identifies PTPN12 as a critical regulator of EGFR endocytosis"**
- **作者**: Jianmin Zhang et al.
- **摘要**: 利用CRISPR筛选技术，发现PTPN12通过调控EGFR的内吞和溶酶体降解，影响EGFR信号通路的持续性，为靶向EGFR的癌症治疗提供新机制。
4. **"PTPN12 loss promotes genomic instability in triple-negative breast cancer"**
- **作者**: Jean Zhao et al.
- **摘要**: 该研究证明PTPN12在三阴性乳腺癌中通过稳定PLK1激酶维持基因组稳定性，其缺失导致染色体错误分离和肿瘤恶性表型增强。
以上文献涵盖PTPN12在信号通路调控、肿瘤抑制及基因组稳定性中的关键作用，涉及不同癌症类型及分子机制。
The PTPN12 antibody targets the protein tyrosine phosphatase non-receptor type 12 (PTPN12), also known as PTP-PEST, a member of the protein tyrosine phosphatase (PTP) family. PTPN12 is a cytosolic enzyme that regulates cellular signaling by dephosphorylating tyrosine residues on various substrates, counterbalancing the activity of protein tyrosine kinases (PTKs). It plays a critical role in modulating cell adhesion, migration, and proliferation by interacting with key signaling molecules, including focal adhesion kinases (FAK), paxillin, and Cas. Structurally, PTPN12 contains an N-terminal catalytic domain and a C-terminal region rich in proline, glutamic acid, serine, and threonine (PEST) motifs, which mediate protein-protein interactions and substrate recognition.
Research highlights PTPN12's role as a tumor suppressor, particularly in breast and ovarian cancers. Its loss or inactivation is linked to enhanced oncogenic signaling, such as hyperactivation of receptor tyrosine kinases (RTKs) or Src family kinases, promoting metastasis and chemoresistance. PTPN12 antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in clinical samples or experimental models. Validating these antibodies involves confirming specificity through knockdown or knockout controls, ensuring accurate detection in diverse biological systems. Ongoing studies aim to elucidate PTPN12's therapeutic potential as a biomarker or target in cancer and inflammatory diseases.
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