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Rabbit Polyclonal PHC1(N-Term) Antibody

  • 中文名: PHC1 (N-Term)抗体
  • 别    名: Polyhomeotic-like protein 1, hPH1, Early development regulatory protein 1, PHC1, EDR1, PH1
货号: IPDX33542
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPolyhomeotic-like protein 1, hPH1, Early development regulatory protein 1, PHC1, EDR1, PH1
Entrez GeneID1911
WB Predicted band size105.5kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis PHC1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 308-342 amino acids from human PHC1.

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参考文献

以下是关于PHC1 (N-Term)抗体的**模拟参考文献示例**(仅供参考,实际文献需通过学术数据库查询):

1. **文献名称**:*"Polyhomeotic Homolog 1 (PHC1) Cooperates with PRC1 to Maintain Embryonic Stem Cell Identity"*

**作者**:Zhang Y, et al.

**摘要**:本研究利用PHC1 (N-Term)抗体进行染色质免疫沉淀(ChIP),揭示了PHC1通过N端结构域与PRC1复合体结合,调控胚胎干细胞多能性相关基因的沉默。

2. **文献名称**:*"N-Terminal Antibody-Based Detection of PHC1 Dysregulation in Breast Cancer"*

**作者**:Lee S, et al.

**摘要**:通过PHC1 (N-Term)抗体的Western blot和免疫组化分析,发现PHC1在乳腺癌组织中表达异常,且其N端结构域缺失与患者预后不良相关。

3. **文献名称**:*"Structural Insights into PHC1 N-Terminal Domain Mediating Chromatin Compaction"*

**作者**:Garcia-Teruel M, et al.

**摘要**:结合X射线晶体学和PHC1 (N-Term)抗体的功能实验,阐明PHC1的N端区域在PRC1介导的染色质凝聚中的关键作用。

4. **文献名称**:*"PHC1 Knockout Mouse Model Reveals Neural Tube Defects via Altered Histone Modification"*

**作者**:Chen R, et al.

**摘要**:利用PHC1 (N-Term)抗体检测小鼠胚胎组织,证实PHC1缺失导致H2A泛素化水平下降,进而引发神经管闭合异常。

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**注意**:以上为模拟示例,实际文献需通过**PubMed**、**Web of Science**等平台以关键词“PHC1 antibody N-terminal”或“PHC1 N-Term epitope”检索。如需具体文献,建议提供更详细的研究背景或应用场景。

背景信息

The PHC1 (N-Term) antibody targets the N-terminal region of Polyhomeotic Homolog 1 (PHC1), a core component of the Polycomb Repressive Complex 1 (PRC1). PHC1 is part of the Polycomb group (PcG) protein family, which plays a critical role in epigenetic gene silencing and chromatin remodeling. PRC1. along with PRC2. maintains transcriptional repression of developmental regulators, particularly through histone modifications. PRC1 facilitates monoubiquitination of histone H2A at lysine 119 (H2AK119ub), promoting chromatin compaction and stabilizing gene silencing. PHC1 contributes to PRC1 assembly and its recruitment to target loci, often through interactions with other PcG proteins or non-coding RNAs. The N-terminal domain of PHC1 contains a conserved sterile alpha motif (SAM) involved in protein oligomerization, which is essential for forming higher-order PRC1 complexes and mediating long-range chromatin interactions. Dysregulation of PHC1/PRC1 activity is linked to developmental disorders, cancer progression, and stem cell pluripotency. The PHC1 (N-Term) antibody is widely used in research to study PRC1-dependent epigenetic mechanisms, detect PHC1 expression in cellular or tissue samples (via techniques like Western blot, immunohistochemistry, or ChIP-seq), and explore its role in maintaining cellular identity or driving disease. Its specificity for the N-terminal region ensures recognition of full-length or truncated PHC1 isoforms, aiding functional studies in developmental biology and oncology.

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