Rabbit Polyclonal MouseSirt1 Antibody

The mouse Sirt1 antibody is a research tool designed to detect and analyze the expression of Sirtuin 1 (Sirt1), a NAD+-dependent deacetylase involved in critical cellular processes such as aging, metabolism, DNA repair, and stress response. Sirt1. a member of the sirtuin family, regulates gene silencing, mitochondrial biogenesis, and energy homeostasis by deacetylating histone and non-histone targets, including transcription factors like p53. FOXOs, and PGC-1α. This antibody specifically targets murine Sirt1. enabling researchers to study its localization, expression levels, and interactions in mouse-derived tissues or cell lines. It is commonly used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Validation typically includes testing in Sirt1 knockout or knockdown models to confirm specificity. The antibody aids in exploring Sirt1's roles in age-related diseases, metabolic disorders, and cancer, as well as its modulation by dietary interventions (e.g., caloric restriction) or pharmacological agents. Proper controls, such as isotype-matched antibodies and sample pretreatment with siRNA, are essential to ensure data reliability. Commercial variants may differ in host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and epitope recognition (C-terminal vs. N-terminal regions).