| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cadherin-15, Cadherin-14, Muscle cadherin, M-cadherin, CDH15, CDH14, CDH3 |
| Entrez GeneID | 1013 |
| WB Predicted band size | 88.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This M Cadherin (CDH15) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 739-767 amino acids from the C-terminal region of human M Cadherin (CDH15). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于M Cadherin (CDH15)抗体的参考文献摘要概括：
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1. **文献名称**: "CDH15 functions as a novel cell adhesion molecule in skeletal muscle differentiation"
**作者**: Suzuki, S.T., et al.
**摘要**: 该研究通过开发特异性CDH15多克隆抗体，验证其在骨骼肌细胞分化中的表达模式。免疫组化显示CDH15在肌管形成阶段显著上调，提示其在肌细胞粘附中的关键作用。
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2. **文献名称**: "Monoclonal antibody targeting CDH15 inhibits epithelial-mesenchymal transition in colorectal cancer"
**作者**: Zhang, Y., et al.
**摘要**: 研究团队制备了一种高特异性抗CDH15单克隆抗体，并通过Western blot和免疫荧光验证其在结直肠癌细胞中的靶向性。实验表明，抗体可阻断CDH15介导的细胞迁移，抑制肿瘤转移。
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3. **文献名称**: "CDH15 expression in developing mouse brain revealed by a novel antibody-based mapping approach"
**作者**: Thompson, R.P., & Lee, K.
**摘要**: 利用新开发的CDH15抗体进行小鼠脑组织免疫染色，揭示了其在胚胎期神经嵴细胞中的动态表达，提示CDH15参与神经管闭合和神经细胞迁移调控。
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(注：以上为示例性内容，实际文献需通过数据库检索确认。)
M Cadherin (CDH15), a member of the cadherin superfamily of calcium-dependent cell adhesion molecules, plays a critical role in mediating cell-cell interactions during tissue morphogenesis and maintenance. Primarily expressed in myoblasts, skeletal muscle, and certain neural tissues, CDH15 is classified as a "classical" type II cadherin due to its structural homology and conserved extracellular cadherin repeats. It facilitates homophilic binding, promoting adhesion between cells to regulate skeletal muscle development, neuromuscular junction formation, and neuronal circuit organization. Dysregulation of CDH15 has been implicated in pathological conditions, including muscle dystrophies, metastatic cancers (e.g., colon cancer, rhabdomyosarcoma), and neurodevelopmental disorders.
Antibodies targeting CDH15 are essential tools for investigating its expression, localization, and function in both physiological and disease contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels in tissue samples or cell cultures. Many commercially available CDH15 antibodies are raised against specific epitopes within its extracellular or cytoplasmic domains, with validation data confirming reactivity across human, mouse, and rat homologs. Researchers also utilize CDH15 antibodies to study its role in epithelial-mesenchymal transition (EMT), cancer metastasis, and muscle regeneration models. Quality control for these reagents often includes knockout validation and cross-reactivity screens to ensure specificity, given the structural similarities among cadherin family members.
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