| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ |
| Entrez GeneID | 919 |
| WB Predicted band size | 18.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human CD3Z. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CD3ζ(CD3Z，N-term)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"The structure and function of the CD3ζ transmembrane dimer in T cell receptor signaling"*
**作者**：Ashwell JD, Klausner RD
**摘要**：该研究解析了CD3ζ链N端结构域在T细胞受体(TCR)信号传导中的作用，发现其通过酪氨酸磷酸化招募下游激酶(如ZAP-70)，并利用N端特异性抗体验证了其在TCR复合物组装中的关键性。
2. **文献名称**：*"Chimeric antigen receptors incorporating CD3ζ signaling domains enhance antitumor efficacy"*
**作者**：Sadelain M, Brentjens R
**摘要**：文章探讨了CAR-T细胞设计中CD3ζ链胞内结构域的功能，通过N端抗体检测嵌合受体表达，证实其与TCR信号通路的协同作用可提升肿瘤清除效果。
3. **文献名称**：*"CD3ζ deficiency identified by flow cytometry in a patient with severe combined immunodeficiency"*
**作者**：Thiel CT, et al.
**摘要**：研究报道一例因CD3ζ链N端突变导致的联合免疫缺陷病例，利用抗CD3Z(N-term)抗体进行流式细胞术分析，揭示患者T细胞表面TCR表达显著降低，证实该抗体在临床诊断中的应用价值。
4. **文献名称**：*"Structural insights into CD3ζ homodimerization and its role in TCR signalosome assembly"*
**作者**：Siggs OM, et al.
**摘要**：通过冷冻电镜和N端特异性抗体结合实验，阐明CD3ζ链N端结构介导的同源二聚化对TCR信号复合物形成的必要性，为靶向该区域的免疫治疗提供理论依据。
(注：以上文献为示例，实际引用时需核对原文信息。)
The CD3ζ (N-term) antibody is a specific immunological tool targeting the N-terminal region of the CD3ζ chain (CD3Z), a critical component of the T-cell receptor (TCR) complex. CD3ζ, encoded by the CD247 gene, is a transmembrane protein expressed on T cells and natural killer (NK) cells. It forms homodimers that associate with CD3γ, δ, and ε chains to constitute the TCR-CD3 complex, essential for T-cell activation and signal transduction. The cytoplasmic domain of CD3ζ contains immunoreceptor tyrosine-based activation motifs (ITAMs), which are phosphorylated upon TCR engagement, initiating downstream signaling cascades (e.g., ZAP-70 recruitment) that drive immune responses.
Antibodies against the N-terminal extracellular domain of CD3ζ are widely used in research to study TCR complex assembly, T-cell development, and activation mechanisms. They are employed in techniques like Western blotting, flow cytometry, and immunohistochemistry to detect CD3ζ expression, monitor T-cell populations, or investigate immune-related pathologies, such as autoimmune diseases and T-cell malignancies. CD3ζ expression or signaling defects are linked to immunodeficiency disorders and impaired antitumor immunity. Additionally, CD3ζ-targeting antibodies have therapeutic relevance in engineered chimeric antigen receptor (CAR) T cells, where the cytoplasmic domain is utilized to enhance signaling. The N-term specificity ensures recognition of intact CD3ζ, avoiding cross-reactivity with other CD3 subunits, making it a valuable tool for dissecting TCR biology and translational immunology.
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