Rabbit Polyclonal NPRL3(N-Term) Antibody

The NPRL3 (N-Term) antibody is a tool designed to detect the N-terminal region of the Nitrogen Permease Regulator-Like 3 (NPRL3) protein, a key component of the GATOR1 complex. This complex acts as a negative regulator of the mTORC1 signaling pathway, which governs cell growth, metabolism, and autophagy in response to nutrient availability. NPRL3. through its interaction with DEPDC5 and NPRL2 in the GATOR1 complex, inhibits mTORC1 activity by promoting its translocation to lysosomal membranes under nutrient-deprived conditions. Antibodies targeting the N-terminal region are particularly useful for studying protein-protein interactions, complex formation, and cellular localization. NPRL3 dysfunction is linked to cancer (e.g., lung, ovarian) and neurological disorders like familial focal epilepsy. Researchers use this antibody in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate NPRL3 expression patterns, its role in mTOR pathway regulation, and disease-associated mutations. Validations typically include testing on knockout cell lines or tissues to confirm specificity. The antibody’s utility extends to both basic research and clinical studies exploring therapeutic targets for mTOR-related pathologies.