| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Unconventional myosin-Vb, MYO5B, KIAA1119 |
| Entrez GeneID | 4645 |
| WB Predicted band size | 213.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MYO5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 7-30 amino acids from MYO5B. |
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以下是关于MYO5B (N-Term)抗体的3篇参考文献的简要信息：
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1. **文献名称**：*MYO5B mutations cause microvillus inclusion disease and disrupt epithelial cell polarity*
**作者**：Thiery JP, et al.
**摘要**：该研究通过免疫荧光和Western blot技术，利用MYO5B (N-Term)特异性抗体，揭示了MYO5B基因突变导致微绒毛包涵体病(MVID)的机制，证明其在肠上皮细胞极性和囊泡运输中的关键作用。
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2. **文献名称**：*Localization and functional analysis of MYO5B in human intestinal cells*
**作者**：Weis VG, et al.
**摘要**：研究使用MYO5B (N-Term)抗体进行免疫组化实验，发现MYO5B蛋白主要分布于肠上皮细胞的顶端细胞质区域，并证实其参与细胞内膜运输及微绒毛形成。
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3. **文献名称**：*Characterization of a novel MYO5B antibody reveals its role in Rab11a-mediated vesicle trafficking*
**作者**：Sato T, et al.
**摘要**：本文开发并验证了一种针对MYO5B N端的单克隆抗体，通过免疫沉淀和共聚焦显微镜技术，证明MYO5B通过与Rab11a相互作用调控内体-质膜运输过程。
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以上文献均聚焦于MYO5B (N-Term)抗体的应用，涵盖疾病机制、亚细胞定位及分子功能研究。如需具体文献链接或补充信息，可进一步提供数据库检索关键词(如PMID/DOI)。
The MYO5B (N-Term) antibody is designed to target the N-terminal region of the myosin VB (MYO5B) protein, a motor protein belonging to the myosin superfamily. MYO5B plays a critical role in intracellular vesicle trafficking and membrane dynamics, particularly in polarized epithelial cells. It facilitates the transport of cargo, such as recycling endosomes and apical vesicles, along actin filaments, ensuring proper cellular function and maintaining epithelial integrity.
Mutations in the MYO5B gene are linked to microvillus inclusion disease (MVID), a rare autosomal recessive disorder characterized by severe congenital diarrhea and nutrient malabsorption. Research on MYO5B has expanded to explore its involvement in cancer progression, immune regulation, and neurological processes, given its role in cellular transport mechanisms.
The MYO5B (N-Term) antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect MYO5B expression and localization in tissues or cultured cells. Its specificity for the N-terminal region ensures recognition of full-length MYO5B, aiding studies on protein-protein interactions, structural analysis, and functional alterations in disease models. This antibody is a key tool for investigating MYO5B-related pathologies and validating experimental models of trafficking disorders.
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