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Rabbit Polyclonal ANO10 Antibody

  • 中文名: ANO10抗体
  • 别    名: Anoctamin-10, Transmembrane protein 16K, ANO10, TMEM16K
货号: IPDX33472
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000-1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAnoctamin-10, Transmembrane protein 16K, ANO10, TMEM16K
Entrez GeneID55129
WB Predicted band size76.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis ANO10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 620-653 amino acids from human ANO10.

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参考文献

以下是关于ANO10抗体的3篇参考文献及其摘要内容的简要概括:

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1. **文献名称**:*ANO10 mutations cause recessive ataxia and cerebellar atrophy via loss of functional protein*

**作者**:Vermeer, S. et al. (2010)

**摘要**:该研究首次报道了ANO10基因突变与常染色体隐性脊髓小脑性共济失调(SCAR10)的关联。研究者通过生成特异性ANO10抗体,证实了ANO10蛋白在小脑浦肯野细胞和颗粒神经元中的表达,并发现患者来源的细胞中ANO10蛋白水平显著降低,提示突变导致功能丧失。

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2. **文献名称**:*ANO10 regulates lipid scrambling and ion transport in cellular membranes*

**作者**:Jiang, D. et al. (2017)

**摘要**:本研究探讨了ANO10作为钙离子激活的脂质 scramblase和氯离子通道的双重功能。通过Western blot和免疫荧光技术(使用ANO10抗体),作者发现ANO10在质膜和内体系统中的定位,并证明其功能缺失导致细胞内离子稳态紊乱,可能与神经退行性疾病相关。

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3. **文献名称**:*Impaired cerebellar development and motor deficits in ANO10-deficient mice*

**作者**:Becker, L. et al. (2018)

**摘要**:研究团队构建了ANO10敲除小鼠模型,利用免疫组化(基于ANO10抗体)观察到小脑浦肯野细胞发育异常和凋亡增加。行为学实验显示小鼠出现共济失调表型,支持ANO10在神经元存活和运动协调中的关键作用。

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如需更具体的研究细节或更多文献,建议通过PubMed或Google Scholar以“ANO10 antibody”或“ANO10 function”为关键词进一步检索。

背景信息

The ANO10 antibody targets the ANO10 protein, also known as transmembrane member 16K (TMEM16K), encoded by the ANO10 gene. This protein belongs to the anoctamin family of calcium-activated chloride channels (CaCCs) involved in ion transport, lipid scrambling, and cellular processes like secretion and apoptosis. ANO10 is primarily expressed in the brain, particularly cerebellar Purkinje cells, and plays roles in regulating intracellular calcium signaling and neuronal excitability. Mutations in the ANO10 gene are linked to autosomal recessive spinocerebellar ataxia type 10 (SCAR10), a neurodegenerative disorder characterized by progressive motor coordination deficits. ANO10 antibodies are critical tools for studying the protein's expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate ANO10's role in cerebellar degeneration, cancer (e.g., colorectal and breast tumors), and other diseases associated with calcium dysregulation. Research utilizing ANO10 antibodies has also explored its interaction with other anoctamins and potential therapeutic targets for related disorders. Validating antibody specificity remains essential due to structural similarities among anoctamin family members.

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