| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Exosome complex component MTR3, Exosome component 6, mRNA transport regulator 3 homolog, hMtr3, p11, EXOSC6, MTR3 |
| Entrez GeneID | 118460 |
| WB Predicted band size | 28.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This EXOSC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-38 amino acids from the N-terminal region of human EXOSC6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EXOSC6 (N-term)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
1. **"The exosome: a conserved eukaryotic RNA processing complex containing multiple 3′→5′ exoribonucleases"**
- **作者**: Allmang C, et al.
- **摘要**: 该研究系统分析了外切体复合体的组成和功能，通过生成针对EXOSC6 N端的抗体，验证了其在RNA加工中的关键作用，并用于亚细胞定位和蛋白质互作实验。
2. **"Dis3-like 1: a novel exoribonuclease associated with the human exosome"**
- **作者**: Staals RH, et al.
- **摘要**: 研究发现Dis3L1与外切体复合体的相互作用，利用EXOSC6 (N-term)抗体进行免疫沉淀和Western blot，揭示其在RNA降解通路中的调控机制。
3. **"EXOSC6 mutations impair RNA exosome function and cause neurodegeneration"**
- **作者**: Wan J, et al.
- **摘要**: 通过EXOSC6 N端特异性抗体检测患者细胞中蛋白表达水平，发现突变导致外切体功能缺陷，与神经退行性疾病相关。
4. **"Structural and functional characterization of the human exosome component EXOSC6"**
- **作者**: Tomecki R, et al.
- **摘要**: 结合晶体结构分析和EXOSC6 N端抗体实验，阐明该亚基在外切体组装及底物识别中的分子机制。
以上文献均涉及EXOSC6 N端抗体的应用，涵盖功能验证、疾病关联及结构研究。实际引用时建议核对文献准确性。
The EXOSC6 (N-term) antibody is a tool designed to detect the N-terminal region of the Exosome Component 6 (EXOSC6) protein, a critical subunit of the RNA exosome complex. The RNA exosome is a multi-protein complex involved in processing, quality control, and degradation of various RNA species, including rRNA, snoRNA, and mRNAs. EXOSC6. also known as MTH4 or p5. forms part of the conserved nine-subunit exosome core, which provides structural stability and 3′→5′ exoribonuclease activity. The N-terminal region of EXOSC6 is essential for interactions with other exosome subunits, such as EXOSC2 and EXOSC4. contributing to the complex’s assembly and function.
This antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study endogenous EXOSC6 expression, localization, and dynamics in cellular models or tissues. Its specificity is validated through knockdown/knockout controls or peptide blocking assays, ensuring minimal cross-reactivity. EXOSC6 has been implicated in diverse biological processes, including ribosome biogenesis, cell proliferation, and stress responses. Dysregulation of EXOSC6 is linked to human diseases, such as pontocerebellar hypoplasia type 1 (PCH1), a severe neurodegenerative disorder caused by EXOSC6 mutations. Additionally, altered exosome activity is associated with cancer progression, making EXOSC6 a potential biomarker or therapeutic target. Researchers utilize this antibody to explore EXOSC6’s role in RNA metabolism, disease mechanisms, and its interplay with other exosome cofactors like RRP6 or DIS3.
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