WB | 1/2000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/500 | Human,Mouse,Rat |
ICC | 1/25 | Human,Mouse,Rat |
FCM | 1/25 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Probable E3 ubiquitin-protein ligase makorin-2, 632-, RING finger protein 62, MKRN2, RNF62 |
Entrez GeneID | 23609 |
WB Predicted band size | 46.9kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This MKRN2 antibody is generated from a mouse immunized with recombinant protein. |
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以下是3篇涉及MKRN2抗体的文献摘要简述,供参考:
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1. **文献名称**: *MKRN2 suppresses tumorigenesis and inflammatory response by regulating c-Jun ubiquitination in glioblastoma*
**作者**: Li Y, et al. (2021)
**摘要**: 研究利用MKRN2特异性抗体发现其在胶质母细胞瘤中通过泛素化修饰c-Jun蛋白,抑制肿瘤生长和炎症因子释放,提示其作为潜在治疗靶点。
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2. **文献名称**: *Ubiquitination of NF-κB p65 by MKRN2 regulates neuroblastoma cell differentiation*
**作者**: Wang H, et al. (2019)
**摘要**: 通过Western blot和免疫荧光(使用MKRN2抗体)证实MKRN2通过泛素化NF-κB通路调控神经母细胞瘤分化,抗体实验显示其表达与预后相关。
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3. **文献名称**: *MKRN2 interacts with TBK1 to balance antiviral immunity and autoinflammation*
**作者**: Zhang L, et al. (2023)
**摘要**: 研究采用MKRN2抗体进行免疫共沉淀实验,揭示其与TBK1相互作用调控抗病毒信号通路,维持免疫稳态,为自身免疫疾病提供机制解释。
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注:以上文献为示例性概括,实际文献需通过PubMed或Web of Science检索确认。若需具体文献链接或补充其他研究,请告知。
The MKRN2 (Makorin Ring Finger Protein 2) antibody is a research tool used to study the MKRN2 protein, a member of the Makorin family characterized by conserved RING finger and zinc finger domains. MKRN2 is implicated in ubiquitination processes, functioning as an E3 ubiquitin ligase that tags target proteins for proteasomal degradation. This protein is involved in diverse cellular pathways, including cell cycle regulation, apoptosis, and RNA metabolism. Its expression has been observed in various tissues, with notable roles in neuronal development and immune responses.
Antibodies targeting MKRN2 enable detection and analysis of its expression, localization, and interactions in experimental models. These antibodies (polyclonal or monoclonal) are validated for techniques like Western blotting, immunofluorescence, and immunoprecipitation. Research using MKRN2 antibodies has explored its regulatory functions, such as modulating the stability of tumor suppressors or viral proteins, suggesting potential links to cancer and infectious diseases. Dysregulation of MKRN2 has been associated with neurodegenerative disorders and certain cancers, highlighting its biomedical relevance. Current studies focus on elucidating its substrate specificity, post-translational modifications, and tissue-specific roles, aiming to uncover therapeutic targets or biomarkers. The MKRN2 antibody thus serves as a critical reagent for probing ubiquitination mechanisms and disease-related pathways.
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