Mouse Monoclonal MKRN2 Antibody

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     All lanes : Anti-MKRN2 Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling MKRN2 with P33457 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG   secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing K562 cells stained with P33457(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33457,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P33457 staining MKRN2 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The MKRN2 (Makorin Ring Finger Protein 2) antibody is a research tool used to study the MKRN2 protein, a member of the Makorin family characterized by conserved RING finger and zinc finger domains. MKRN2 is implicated in ubiquitination processes, functioning as an E3 ubiquitin ligase that tags target proteins for proteasomal degradation. This protein is involved in diverse cellular pathways, including cell cycle regulation, apoptosis, and RNA metabolism. Its expression has been observed in various tissues, with notable roles in neuronal development and immune responses.

Antibodies targeting MKRN2 enable detection and analysis of its expression, localization, and interactions in experimental models. These antibodies (polyclonal or monoclonal) are validated for techniques like Western blotting, immunofluorescence, and immunoprecipitation. Research using MKRN2 antibodies has explored its regulatory functions, such as modulating the stability of tumor suppressors or viral proteins, suggesting potential links to cancer and infectious diseases. Dysregulation of MKRN2 has been associated with neurodegenerative disorders and certain cancers, highlighting its biomedical relevance. Current studies focus on elucidating its substrate specificity, post-translational modifications, and tissue-specific roles, aiming to uncover therapeutic targets or biomarkers. The MKRN2 antibody thus serves as a critical reagent for probing ubiquitination mechanisms and disease-related pathways.