| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphatidylinositol 3,5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3,5)-dependent Rac exchanger 1, PREX1, KIAA1415 |
| Entrez GeneID | 57580 |
| WB Predicted band size | 186.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PREX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 567-594 amino acids from the Central region of human PREX1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PREX1抗体的3篇参考文献示例，涵盖其在不同研究中的应用和发现：
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1. **文献名称**：*PREX1 integrates G protein-coupled receptor and phosphoinositide 3-kinase signaling to promote Rac activation and cell migration*
**作者**：Montero JC, et al.
**摘要**：该研究揭示了PREX1通过整合GPCR和PI3K信号通路激活Rac GTP酶，进而促进癌细胞迁移。实验中利用特异性PREX1抗体进行免疫沉淀和Western blot分析，证实了PREX1在乳腺癌细胞侵袭中的关键作用。
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2. **文献名称**：*Targeting PREX1 inhibits mitochondrial metabolism and radiosensitizes triple-negative breast cancer cells*
**作者**：Cairns RA, et al.
**摘要**：文章探讨了PREX1在三阴性乳腺癌中对线粒体代谢的调控作用。通过使用PREX1抗体敲低蛋白表达，研究发现抑制PREX1可增强肿瘤细胞对放疗的敏感性，为靶向治疗提供了新思路。
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3. **文献名称**：*The Rac-GEF PREX1 contributes to the tumor microenvironment by regulating macrophage infiltration*
**作者**：Snyder JC, et al.
**摘要**：该研究通过免疫组化结合PREX1抗体，发现肿瘤微环境中PREX1高表达与巨噬细胞浸润相关，提示其在免疫逃逸中的潜在作用，为癌症免疫治疗提供了新靶点。
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4. **文献名称**：*Structural insights into the autoinhibition of PREX1 and its activation by Gβγ subunits*
**作者**：Lyon AM, et al.
**摘要**：利用冷冻电镜和抗体标记技术，解析了PREX1的自抑制构象及其被Gβγ亚基激活的分子机制，为开发靶向PREX1-Rac信号通路的小分子抑制剂奠定结构基础。
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这些文献展示了PREX1抗体在分子机制研究、癌症治疗策略探索及结构生物学中的应用。实际检索时建议通过PubMed或Google Scholar以“PREX1 antibody”为关键词获取最新研究。
PREX1 (Phosphatidylinositol-3.4.5-Trisphosphate Dependent Rac Exchange Factor 1) is a guanine nucleotide exchange factor (GEF) that activates Rac GTPases, critical regulators of cytoskeletal dynamics, cell migration, and intracellular signaling. The PREX1 antibody is a research tool designed to detect and quantify PREX1 protein expression in various experimental settings, such as Western blotting, immunohistochemistry, or immunofluorescence.
PREX1 gained attention due to its role in cancer progression, particularly in breast cancer and melanoma, where it promotes metastasis by enhancing cell motility and invasion. It interacts with PTEN (phosphatase and tensin homolog), a tumor suppressor, and its overexpression is linked to PTEN loss-driven tumorigenesis. Additionally, PREX1 is implicated in neurological disorders and immune regulation, highlighting its broad biological relevance.
Antibodies targeting PREX1 are essential for studying its expression patterns, subcellular localization, and interactions in disease models. Many commercial PREX1 antibodies are raised against specific epitopes, often within its N-terminal or C-terminal regions, and vary in host species (e.g., rabbit, mouse). Validation remains crucial, as PREX1 shares homology with PREX2. potentially causing cross-reactivity. Researchers frequently employ these antibodies to explore PREX1's mechanistic contributions to pathologies or its utility as a biomarker, though challenges in specificity and reproducibility underscore the need for rigorous antibody validation.
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