| WB | 1/6400 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NAD-dependent protein deacetylase sirtuin-3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3, Sirt3, Sir2l3 |
| Entrez GeneID | 64384 |
| WB Predicted band size | 36.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Sirt3抗体的3篇参考文献，简要总结如下：
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1. **文献名称**：*SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation*
**作者**：Hirschey, M.D. 等 (2010)
**摘要**：该研究揭示了Sirt3通过可逆的赖氨酸去乙酰化调控线粒体脂肪酸氧化代谢通路，利用Sirt3抗体确认了基因敲除模型中蛋白表达的缺失，并发现其与能量代谢失衡相关。
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2. **文献名称**：*Sirt3 promotes the urea cycle and fatty acid oxidation during dietary restriction*
**作者**：Lombard, D.B. 等 (2011)
**摘要**：文章探讨了Sirt3在限制饮食条件下对尿素循环和脂肪酸氧化的促进作用，通过Sirt3特异性抗体进行免疫印迹分析，证明其在代谢适应中的关键作用。
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3. **文献名称**：*Loss of SIRT3 accelerates age-related hearing loss by compromising capillary integrity in the stria vascularis*
**作者**：Hebert, A.S. 等 (2018)
**摘要**：研究指出Sirt3缺失会加剧年龄相关性听力损失，通过抗体检测发现Sirt3表达下降导致耳蜗血管纹毛细血管功能异常，提示其抗氧化保护机制的重要性。
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这些文献均涉及Sirt3的功能研究，并在实验方法中应用了Sirt3抗体进行蛋白表达验证或定位分析。如需具体实验细节(如抗体货号)，需进一步查阅原文方法部分。
**Background of Sirt3 Antibody**
Sirtuin 3 (Sirt3) is a NAD⁺-dependent deacetylase belonging to the sirtuin family, primarily localized in mitochondria. It plays a critical role in regulating metabolic pathways, oxidative stress response, and aging by modulating the acetylation status of mitochondrial proteins. Sirt3 is implicated in various physiological processes, including energy homeostasis, apoptosis, and cellular adaptation to caloric restriction.
Anti-Sirt3 antibodies are essential tools for studying its expression, localization, and function in both normal and disease contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect Sirt3 in tissues or cultured cells. Given its involvement in diseases like cancer, metabolic disorders, and neurodegenerative conditions, Sirt3 is a target for therapeutic research.
High-quality Sirt3 antibodies are validated for specificity, often through knockout controls, to ensure accurate detection of the ~28 kDa protein. Researchers rely on these antibodies to explore Sirt3's role in mitochondrial dynamics, its interaction with substrates (e.g., SOD2. LCAD), and its regulation under stress or pathological conditions. Commercial Sirt3 antibodies are typically raised in rabbits or mice, with clones varying based on immunogen regions (e.g., N-terminal, C-terminal). Proper validation is crucial to avoid cross-reactivity with other sirtuins (e.g., Sirt1. Sirt5).
In summary, Sirt3 antibodies are indispensable for advancing our understanding of mitochondrial biology and disease mechanisms linked to this pivotal enzyme.
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