| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46, LDHB |
| Entrez GeneID | 3945 |
| WB Predicted band size | 36.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 220-250 amino acids of human. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是针对HUMAN-LDHB(Y240)抗体的模拟参考文献示例(实际文献可能需要通过学术数据库验证)：
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1. **"Phosphorylation of LDHB at tyrosine 240 regulates cancer cell glycolysis"**
*Authors: Chen X, Wang Y, et al.*
摘要：研究揭示了LDHB在Y240位点的磷酸化修饰通过增强酶活性促进肿瘤细胞糖酵解，开发的Y240特异性抗体用于检测其在结直肠癌中的表达及临床意义。
2. **"Development of a monoclonal antibody targeting human LDHB phosphorylated Y240 for metabolic studies"**
*Authors: Tanaka K, Suzuki H, et al.*
摘要：本文报道了一种高特异性抗体的制备，该抗体可识别LDHB Y240磷酸化形式，应用于肝癌模型中以研究乳酸代谢与化疗耐药性的关联。
3. **"Site-specific modification of LDHB regulates its interaction with HIF-1α in hypoxic tumors"**
*Authors: Liu R, Zhang P, et al.*
摘要：利用Y240磷酸化抗体，研究发现缺氧条件下LDHB与HIF-1α的相互作用增强，提示该位点修饰在肿瘤微环境适应中的关键作用。
4. **"Validation of a novel LDHB(Y240) antibody for immunohistochemical applications"**
*Authors: Müller S, Braun G, et al.*
摘要：验证了一种商业化LDHB Y240抗体的特异性，证明其在乳腺癌组织芯片中检测氧化应激相关酶活性变化的可靠性。
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**注**：以上为模拟生成的参考文献，实际研究中可能需结合具体修饰类型(如磷酸化)或应用场景检索(推荐使用PubMed/Google Scholar关键词：LDHB Y240 phosphorylation antibody)。
The HUMAN-LDHB(Y240) antibody is a specific monoclonal or polyclonal antibody designed to detect the human lactate dehydrogenase B (LDHB) protein, particularly targeting the tyrosine residue at position 240 (Y240). LDHB is one of the subunits of lactate dehydrogenase, a key enzyme in anaerobic glycolysis that catalyzes the interconversion of pyruvate and lactate. This isoform is predominantly expressed in metabolically active tissues, such as the heart, liver, and skeletal muscle, and plays a critical role in cellular energy metabolism. The Y240 residue may be associated with post-translational modifications or functional domains influencing enzyme activity, localization, or protein-protein interactions.
LDHB dysregulation has been implicated in various cancers, as altered glycolysis (the Warburg effect) is a hallmark of tumor metabolism. The HUMAN-LDHB(Y240) antibody is widely used in research to investigate LDHB expression patterns, subcellular localization, and its role in cancer progression, metastasis, or therapy resistance. It is validated for applications including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Researchers also utilize this antibody to explore metabolic reprogramming in diseases and evaluate LDHB as a potential biomarker or therapeutic target. Specificity for the Y240 epitope ensures precise detection of LDHB variants or modified forms, aiding mechanistic studies of its regulation in health and disease.
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