| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear receptor coactivator 2, NCoA-2, Class E basic helix-loop-helix protein 75, bHLHe75, Transcriptional intermediary factor 2, hTIF2, NCOA2, BHLHE75, SRC2, TIF2 |
| Entrez GeneID | 10499 |
| WB Predicted band size | 159.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NCOA2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1431-1462 amino acids from human NCOA2. |
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以下是关于NCOA2抗体的3篇示例文献(虚构示例，仅供格式参考)：
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1. **文献名称**：*NCOA2/SRC-2 regulates estrogen receptor signaling in breast cancer progression*
**作者**：Li Y, et al.
**摘要**：本研究利用NCOA2特异性抗体进行免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)，揭示NCOA2通过增强雌激素受体α(ERα)的转录活性促进乳腺癌细胞增殖，为靶向NCOA2的疗法提供依据。
2. **文献名称**：*Characterization of NCOA2 antibody specificity in prostate cancer models*
**作者**：Smith JR, et al.
**摘要**：通过Western blot和免疫组化验证NCOA2抗体的特异性，证明其在人前列腺癌组织中高表达，并与雄激素受体(AR)共定位，提示NCOA2在去势抵抗性前列腺癌中的潜在作用。
3. **文献名称**：*NCOA2 modulates metabolic homeostasis via PPARγ coactivation*
**作者**：Wang H, et al.
**摘要**：采用NCOA2抗体进行组织免疫荧光和RNA干扰实验，发现NCOA2作为PPARγ的共激活因子，调控脂肪细胞分化和能量代谢，缺失NCOA2导致小鼠代谢综合征表型。
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*注：以上文献为示例，实际引用请查询真实数据库(如PubMed)。*
**Background of NCOA2 Antibody**
The nuclear receptor coactivator 2 (NCOA2), also known as glucocorticoid receptor-interacting protein 1 (GRIP1) or transcriptional intermediary factor 2 (TIF2), is a member of the p160/SRC family of coactivators. It plays a critical role in transcriptional regulation by interacting with nuclear receptors (e.g., estrogen, androgen, and thyroid hormone receptors) and other transcription factors. NCOA2 facilitates chromatin remodeling and recruitment of transcriptional machinery through its intrinsic histone acetyltransferase (HAT) activity and by bridging interactions with secondary coactivators like CREB-binding protein (CBP/p300).
NCOA2 antibodies are immunological tools designed to detect and study the expression, localization, and function of the NCOA2 protein in various biological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP). Dysregulation of NCOA2 has been implicated in hormone-dependent cancers (e.g., breast and prostate cancers), metabolic disorders, and developmental abnormalities. Researchers utilize NCOA2 antibodies to investigate its role in signaling pathways, gene regulation, and disease mechanisms.
Most NCOA2 antibodies target specific domains, such as the N-terminal basic helix-loop-helix (bHLH) region or the C-terminal activation domain, ensuring specificity. Validation across species (human, mouse, rat) and applications is essential for experimental reliability. Studies using these antibodies have advanced understanding of NCOA2's contributions to cellular differentiation, proliferation, and hormone responsiveness, making it a focal point in therapeutic and diagnostic research.
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