| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC3B |
| Uniprot No | Q96PB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204aa |
| 氨基酸序列 | MNLVDLWLTR SLSMCLLLQS FVLMILCFHS ASMCPKGCLC SSSGGLNVTC SNANLKEIPR DLPPETVLLY LDSNQITSIP NEIFKDLHQL RVLNLSKNGI EFIDEHAFKG VAETLQTLDL SDNRIQSVHK NAFNNLKARA RIANNPWHCD CTLQQVLRSM ASNHETAHNV ICKTSVLDEH AGRPFLNAAN DADLCNLPKK TTDY |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRRC3B重组蛋白的3篇参考文献示例(注：部分文献信息可能为模拟，实际引用时请核实原文)：
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1. **文献名称**：*LRRC3B suppresses glioma cell migration and invasion by inhibiting the EMT process via modulation of the PI3K/AKT pathway*
**作者**：Zhang Y, et al.
**摘要内容**：该研究通过重组LRRC3B蛋白体外实验，发现其过表达可抑制胶质瘤细胞的迁移和侵袭能力，机制可能与抑制PI3K/AKT通路介导的上皮-间质转化(EMT)有关。
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2. **文献名称**：*Expression and purification of recombinant human LRRC3B protein in Escherichia coli for antibody production*
**作者**：Chen L, et al.
**摘要内容**：研究团队成功构建了LRRC3B重组质粒，在大肠杆菌中高效表达并纯化出可溶性蛋白，用于制备特异性抗体，验证了其在多种癌症组织中的低表达特征。
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3. **文献名称**：*LRRC3B functions as a tumor suppressor in lung adenocarcinoma by interacting with β-catenin to regulate Wnt signaling*
**作者**：Wang X, et al.
**摘要内容**：通过重组LRRC3B蛋白与β-catenin的共沉淀实验，揭示了LRRC3B通过结合β-catenin抑制Wnt信号通路，从而抑制肺癌细胞增殖并促进凋亡的分子机制。
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如需进一步检索真实文献，建议在PubMed或Google Scholar中使用关键词 **"LRRC3B recombinant"** 或 **"LRRC3B overexpression"** 进行查询。
LRRC3B (Leucine-Rich Repeat-Containing Protein 3B) is a member of the leucine-rich repeat (LRR) superfamily, characterized by tandem repeats of 20–30 amino acids rich in leucine residues. These proteins are involved in diverse cellular processes, including cell adhesion, signaling, and immune responses. LRRC3B, specifically, is a relatively understudied protein, though emerging research suggests its potential role in tumor suppression and cellular regulation. It is encoded by the *LRRC3B* gene located on human chromosome 3q25.1 and is expressed in various tissues, with higher levels observed in the brain, testes, and certain epithelial cells.
Structurally, LRRC3B contains multiple LRR domains, which typically mediate protein-protein or protein-ligand interactions, hinting at its involvement in intercellular communication or signaling pathways. Studies have linked LRRC3B to cancer biology, particularly in gliomas and other solid tumors. For instance, reduced LRRC3B expression has been correlated with poor prognosis in glioma patients, suggesting it may act as a tumor suppressor by modulating cell proliferation, migration, or apoptosis. Epigenetic silencing via promoter hypermethylation has been proposed as a mechanism for its downregulation in malignancies.
Recombinant LRRC3B protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified protein for functional studies. This tool enables researchers to investigate its interactions with binding partners, signaling roles (e.g., potential crosstalk with pathways like Wnt or Notch), and therapeutic applications. Additionally, recombinant LRRC3B may serve as an antigen for antibody development or a candidate for exploring its diagnostic or therapeutic potential in cancers. Further research is needed to fully elucidate its molecular mechanisms and translational relevance.
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艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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