| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hydroxycarboxylic acid receptor 1, G-protein coupled receptor 104, G-protein coupled receptor 81, HCAR1, GPR104, GPR81, HCA1 |
| Entrez GeneID | 27198 |
| WB Predicted band size | 39.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GPR81 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-316 amino acids from the C-terminal region of human GPR81. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GPR81抗体的3篇模拟参考文献示例(注：文献为虚构，仅作格式参考)：
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1. **文献名称**: "Development and Validation of a High-Affinity Monoclonal Antibody for GPR81 in Metabolic Studies"
**作者**: Smith A, et al.
**摘要**: 本研究报道了一种新型兔源单克隆抗体的开发，该抗体特异性识别GPR81的胞外结构域。通过免疫印迹和免疫荧光验证，证明其可用于检测脂肪细胞中GPR81的蛋白表达，并揭示乳酸通过GPR81调控脂解作用的分子机制。
2. **文献名称**: "GPR81 Antibody-Based Profiling Identifies Its Role in Tumor Stroma Interaction"
**作者**: Chen L, et al.
**摘要**: 利用商业化GPR81抗体(货号AB123)，研究者通过免疫组化分析发现GPR81在乳腺癌微环境中的成纤维细胞中高表达。功能实验表明，GPR81信号促进肿瘤细胞增殖，提示其作为潜在治疗靶点。
3. **文献名称**: "Comparative Study of GPR81 Antibody Specificity Across Species Using Knockout Models"
**作者**: Tanaka K, et al.
**摘要**: 通过构建GPR81基因敲除小鼠模型，系统性评估了多种市售GPR81抗体的特异性。研究发现部分抗体存在非特异性结合，强调抗体验证在代谢组织(如肝脏和脂肪)研究中的关键性。
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**注**：以上文献为模拟内容，实际研究中请通过PubMed或Google Scholar检索真实文献(关键词如“GPR81 antibody validation”“GPR81 HCAR1”)。真实文献可能包括抗体开发公司(如Cell Signaling Technology)的技术报告或发表于《PLOS ONE》等期刊的文章。
GPR81 (G-protein-coupled receptor 81), also known as HCAR1 (hydroxycarboxylic acid receptor 1), is a member of the G protein-coupled receptor family predominantly expressed in adipose tissue. It functions as a receptor for lactate, a metabolic byproduct, and plays a critical role in regulating lipid metabolism, insulin sensitivity, and energy homeostasis. GPR81 activation suppresses lipolysis in adipocytes, linking metabolic activity to cellular signaling pathways. Its involvement in metabolic disorders, cancer, and inflammatory conditions has drawn significant research interest.
GPR81 antibodies are essential tools for studying the receptor's expression, localization, and function in various tissues. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to detect GPR81 protein levels in experimental models, including human, mouse, and rat samples. Validated antibodies help elucidate GPR81's role in adipose biology, tumor microenvironment modulation (e.g., lactate-driven cancer progression), and metabolic syndromes such as obesity and diabetes. Recent studies also explore its therapeutic potential, with antibodies aiding in drug development targeting GPR81 pathways. Specificity and cross-reactivity validation are crucial, as GPR81 shares structural homology with other hydroxycarboxylic acid receptors (e.g., GPR109A, GPR132). Research using these antibodies continues to uncover novel insights into metabolic regulation and disease mechanisms.
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