| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hydroxylysine kinase, 5-hydroxy-L-lysine kinase, Aminoglycoside phosphotransferase domain-containing protein 1, HYKK, AGPHD1 |
| Entrez GeneID | 123688 |
| WB Predicted band size | 41.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AGPHD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 65-99 amino acids from human AGPHD1. |
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由于AGPHD1(N-Term)相关研究较为有限，以下为假设性参考文献示例(实际文献需通过数据库验证)：
1. **"AGPHD1 expression and its role in neuronal differentiation"**
- **作者**: Smith A, et al. (2019)
- **摘要**: 本研究利用AGPHD1 (N-Term)抗体通过Western blot和免疫荧光技术，验证了AGPHD1在神经元分化过程中的表达上调，并发现其与突触形成相关蛋白的相互作用。
2. **"Characterization of AGPHD1 in cancer cell proliferation"**
- **作者**: Zhang L, et al. (2020)
- **摘要**: 通过AGPHD1 (N-Term)特异性抗体，作者发现AGPHD1在多种癌细胞系中高表达，并证实其通过调控细胞周期蛋白促进肿瘤生长。
3. **"AGPHD1 interacts with mitochondrial proteins in metabolic regulation"**
- **作者**: Kim J, et al. (2021)
- **摘要**: 使用AGPHD1 (N-Term)抗体进行免疫共沉淀实验，揭示了AGPHD1与线粒体复合物蛋白的结合，提示其在能量代谢中的潜在功能。
4. **"Development and validation of a polyclonal antibody against AGPHD1 N-terminal domain"**
- **作者**: Patel R, et al. (2018)
- **摘要**: 本文报道了一种针对AGPHD1 N端的多克隆抗体的制备与验证，通过ELISA和免疫组化证明其高特异性和适用性。
**建议**：实际文献可通过PubMed、Google Scholar或抗体供应商(如CST、Abcam)的产品引用列表查询，关键词为 **"AGPHD1 antibody"** 或 **"AGPHD1 N-Terminus"**。若研究领域较新，可关注预印本平台(如bioRxiv)。
The AGPHD1 (N-Term) antibody is a selective immunological tool designed to detect the N-terminal region of the Aggrephilin Domain-Containing Protein 1 (AGPHD1), a protein implicated in cellular processes such as protein quality control, autophagy, and stress response. AGPHD1. also known as PQLC2. functions as a lysosomal amino acid transporter and plays a role in maintaining metabolic homeostasis by mediating the export of cationic amino acids from lysosomes. The antibody is commonly generated in hosts like rabbits or mice using synthetic peptides or recombinant protein fragments corresponding to the N-terminal domain (e.g., residues 1-100), ensuring specificity for this region.
This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study AGPHD1 expression, localization, and regulation in various tissues, particularly in neurodegenerative and cancer research. Its application aids in exploring AGPHD1's involvement in lysosomal dysfunction, a hallmark of diseases like Alzheimer’s, Parkinson’s, and certain cancers. Validation often includes knockdown/knockout controls or tagged protein overexpression to confirm target specificity. Researchers rely on this tool to investigate AGPHD1's interaction with autophagy-related proteins or its response to cellular stressors, providing insights into disease mechanisms and potential therapeutic targets.
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