WB | 1/2000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, HLF, mHLF, HIF-related factor, HRF, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Epas1, Hif2a |
Entrez GeneID | 13819 |
WB Predicted band size | 96.7kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This Mouse Epas1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 713-747 amino acids from the C-terminal region of Mouse Epas1. |
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以下是关于小鼠EPAS1(HIF-2α)抗体的3篇参考文献,按文献名称、作者和摘要内容概括列出:
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1. **文献名称**:*Endothelial PAS domain protein 1 (EPAS1) is required for angiogenesis in hypoxia-induced retinopathy*
**作者**:Y. Peng et al.
**摘要**:研究利用EPAS1基因敲除小鼠及特异性抗体验证EPAS1在缺氧诱导视网膜血管生成中的作用,发现该抗体通过Western blot和免疫组化检测到EPAS1在血管内皮细胞中的表达上调,证实其参与调控缺氧适应性血管新生。
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2. **文献名称**:*HIF-2α regulates murine hematopoietic stem cell function in hypoxic stress*
**作者**:K. Kobayashi et al.
**摘要**:通过EPAS1/HIF-2α特异性抗体分析小鼠造血干细胞在低氧条件下的功能,发现该抗体在流式细胞术和免疫荧光中有效标记EPAS1蛋白,揭示其在维持干细胞自我更新中的关键作用。
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3. **文献名称**:*EPAS1 modulates the antioxidant response in murine macrophages during ischemia-reperfusion injury*
**作者**:L. Chen et al.
**摘要**:研究使用抗小鼠EPAS1抗体进行ChIP-seq和免疫沉淀实验,证明EPAS1通过调控抗氧化基因(如SOD2)表达减轻缺血再灌注损伤,抗体特异性在基因型验证实验中得到确认。
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**备注**:若需具体抗体货号或实验细节,建议查阅抗体供应商(如Cell Signaling Technology、Abcam)的产品说明书及相关引用文献。
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