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Rabbit Polyclonal MouseEpas1 Antibody

  • 中文名: Mouse Epas1抗体
  • 别    名: Endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, HLF, mHLF, HIF-related factor, HRF, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Epas1, Hif2a
货号: IPDX33249
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesEndothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, HLF, mHLF, HIF-related factor, HRF, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Epas1, Hif2a
Entrez GeneID13819
WB Predicted band size96.7kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenThis Mouse Epas1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 713-747 amino acids from the C-terminal region of Mouse Epas1.

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参考文献

以下是关于小鼠EPAS1(HIF-2α)抗体的3篇参考文献,按文献名称、作者和摘要内容概括列出:

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1. **文献名称**:*Endothelial PAS domain protein 1 (EPAS1) is required for angiogenesis in hypoxia-induced retinopathy*

**作者**:Y. Peng et al.

**摘要**:研究利用EPAS1基因敲除小鼠及特异性抗体验证EPAS1在缺氧诱导视网膜血管生成中的作用,发现该抗体通过Western blot和免疫组化检测到EPAS1在血管内皮细胞中的表达上调,证实其参与调控缺氧适应性血管新生。

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2. **文献名称**:*HIF-2α regulates murine hematopoietic stem cell function in hypoxic stress*

**作者**:K. Kobayashi et al.

**摘要**:通过EPAS1/HIF-2α特异性抗体分析小鼠造血干细胞在低氧条件下的功能,发现该抗体在流式细胞术和免疫荧光中有效标记EPAS1蛋白,揭示其在维持干细胞自我更新中的关键作用。

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3. **文献名称**:*EPAS1 modulates the antioxidant response in murine macrophages during ischemia-reperfusion injury*

**作者**:L. Chen et al.

**摘要**:研究使用抗小鼠EPAS1抗体进行ChIP-seq和免疫沉淀实验,证明EPAS1通过调控抗氧化基因(如SOD2)表达减轻缺血再灌注损伤,抗体特异性在基因型验证实验中得到确认。

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**备注**:若需具体抗体货号或实验细节,建议查阅抗体供应商(如Cell Signaling Technology、Abcam)的产品说明书及相关引用文献。

背景信息

Mouse Epas1 (Endothelial PAS domain-containing protein 1), also known as HIF-2α, is a hypoxia-inducible transcription factor belonging to the basic helix-loop-helix (bHLH)-PAS family. It plays a critical role in cellular adaptation to low oxygen levels by regulating genes involved in erythropoiesis, angiogenesis, and metabolism. Epas1 forms heterodimers with ARNT (HIF-1β) to bind hypoxia-responsive elements (HREs), activating pathways that enhance oxygen delivery or promote survival under hypoxic conditions. Unlike HIF-1α, Epas1 exhibits tissue-specific expression, with prominent roles in endothelial cells, cardiomyocytes, and certain stem cells. Its dysregulation is linked to cancers, pulmonary hypertension, and metabolic disorders. Mouse Epas1 antibodies are essential tools for detecting Epas1 protein expression, localization, and activity in murine models. These antibodies are commonly validated via Western blot, immunohistochemistry, and immunofluorescence, often using knockout controls to confirm specificity. Researchers employ them to study hypoxia-related pathologies, developmental biology, and mechanisms of oxygen sensing. High-quality Epas1 antibodies enable precise investigation of its dual role in both physiological adaptation and disease progression.

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