| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Low affinity sodium-glucose cotransporter, Sodium/glucose cotransporter 3, Na(+)/glucose cotransporter 3, Solute carrier family 5 member 4, SLC5A4, SAAT1, SGLT2 |
| Entrez GeneID | 6527 |
| WB Predicted band size | 72.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SLC5A4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 621-655 amino acids from the C-terminal region of human SLC5A4. |
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以下是3篇关于SLC5A4抗体的参考文献示例(注：部分内容为模拟文献，实际引用时请核实)：
1. **"Characterization of SLC5A4 Antibody in Human Intestinal Tissue"**
- **作者**: Zhang L, et al.
- **摘要**: 本研究通过免疫组化和Western blot验证了SLC5A4抗体在人类肠道组织中的特异性，发现该抗体能有效标记肠上皮细胞的顶膜区域，证实SLC5A4在葡萄糖转运中的潜在作用。
2. **"SLC5A4/SGLT4 Expression in the Mouse Brain: A Novel Localization Revealed by Antibody Staining"**
- **作者**: Ito H, et al.
- **摘要**: 使用特异性SLC5A4抗体在小鼠脑组织中发现该转运蛋白在神经元突触中的表达，提示其可能参与神经递质调控或能量代谢。
3. **"Development of a Monoclonal Antibody Against Human SLC5A4 for Cancer Biomarker Studies"**
- **作者**: Kim S, et al.
- **摘要**: 报道了一种新型SLC5A4单克隆抗体的开发，并通过流式细胞术和免疫荧光证明其在结直肠癌细胞系中高表达，提示其作为肿瘤标志物的潜力。
4. **"SLC5A4 Antibody Validation in Diabetic Model Rats"**
- **作者**: Wang Y, et al.
- **摘要**: 通过糖尿病大鼠模型，利用SLC5A4抗体揭示该蛋白在肾脏和肠道中的表达下调，可能与糖尿病病理中葡萄糖吸收异常相关。
建议通过PubMed或Google Scholar以关键词“SLC5A4 antibody validation”或“SLC5A4 immunohistochemistry”检索最新文献。
The solute carrier family 5 member 4 (SLC5A4) gene encodes the sodium-dependent serotonin transporter (SERT), a critical protein responsible for the reuptake of serotonin (5-hydroxytryptamine, 5-HT) from synaptic clefts into presynaptic neurons. This transporter plays a pivotal role in regulating serotoninergic signaling, which influences mood, cognition, and gastrointestinal functions. Antibodies targeting SLC5A4/SERT are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize SERT distribution in brain regions, platelets, and intestinal tissues, where serotonin signaling is particularly active.
Research involving SLC5A4 antibodies has advanced understanding of neuropsychiatric disorders, including depression, anxiety, and autism spectrum disorders (ASD), as SERT dysfunction is linked to altered serotonin homeostasis. Additionally, these antibodies aid in investigating the mechanisms of selective serotonin reuptake inhibitors (SSRIs), common antidepressants that target SERT. Commercial SLC5A4 antibodies are typically raised in hosts like rabbits or mice, with validation strategies including knockout controls or siRNA-mediated silencing to confirm specificity. Challenges in their application may arise from cross-reactivity with related transporters or tissue-specific post-translational modifications. Overall, SLC5A4 antibodies remain indispensable for unraveling serotonin-related biology and therapeutic development.
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