| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Forkhead box protein H1, Forkhead activin signal transducer 1, Fast-1, Forkhead activin signal transducer 2, Fast-2, Foxh1, Fast1, Fast2 |
| Entrez GeneID | 14106 |
| WB Predicted band size | 44.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Foxh1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 290-322 amino acids from the C-terminal region of Mouse Foxh1. |
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以下是关于Mouse Foxh1抗体的3条参考文献示例，基于学术文献的典型研究内容整理：
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1. **文献名称**：*Foxh1 (FAST) mediates transcriptional responses to Nodal/TGF-β signaling in embryonic stem cells*
**作者**：Hoodless, P.A., et al.
**摘要**：该研究揭示了Foxh1作为TGF-β/Nodal信号通路下游的关键转录调控因子，通过ChIP实验和Western blot分析，使用特异性抗体验证Foxh1在胚胎干细胞中的表达及与靶基因启动子的结合，证实其调控多能性相关基因的功能。
2. **文献名称**：*The role of Foxh1 in cardiac development: Insights from mouse knockout models*
**作者**：von Both, I., et al.
**摘要**：通过构建Foxh1基因敲除小鼠模型，结合免疫组化(使用Foxh1特异性抗体)和原位杂交技术，研究发现Foxh1缺失导致心脏发育缺陷，强调了其在心脏祖细胞分化和心管形成中的关键作用。
3. **文献名称**：*Antibody-based validation of Foxh1 interaction with Smad proteins in TGF-β signaling*
**作者**：Chen, Y., et al.
**摘要**：该研究利用免疫共沉淀(Co-IP)和免疫荧光技术，通过小鼠源Foxh1抗体证实Foxh1与Smad2/3蛋白的相互作用，阐明了其在TGF-β信号转导中的分子机制，并验证了抗体的特异性及适用性。
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这些示例反映了Foxh1抗体在信号通路、发育生物学及蛋白相互作用研究中的应用。如需具体文献，建议通过PubMed或Google Scholar以关键词“Foxh1 antibody”、“Foxh1 mouse model”或“Foxh1 TGF-β”检索近年研究。
The Mouse Foxh1 antibody is a tool used to detect and study the Forkhead box H1 (Foxh1) protein, a transcription factor critical in early embryonic development. Foxh1 belongs to the forkhead family of DNA-binding proteins and acts as a key mediator of Transforming Growth Factor-beta (TGF-β) and Nodal signaling pathways. It binds directly to Smad2/4 complexes activated by these pathways, facilitating transcriptional regulation of target genes involved in mesoderm formation, left-right axis patterning, and anterior-posterior body plan establishment. Studies in mice have shown that Foxh1 deficiency leads to severe developmental defects, including gastrulation failure, highlighting its essential role in embryogenesis.
This antibody is commonly generated in rabbits or mice using immunogenic peptides or recombinant proteins corresponding to conserved regions of the Foxh1 protein. It is widely utilized in techniques like Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP) to assess Foxh1 expression, localization, and DNA-binding activity in cells or tissues. Validation often includes testing in Foxh1-knockout models to confirm specificity. Researchers employ this antibody to investigate Foxh1's interactions with signaling partners, its regulatory mechanisms in stem cell differentiation, and its potential dysregulation in developmental disorders or cancer. Its applications span developmental biology, signal transduction research, and studies of cell fate determination.
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