| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sex-determining region Y protein, Testis-determining factor, SRY, TDF |
| Entrez GeneID | 6736 |
| WB Predicted band size | 23.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SRY antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 52-82 amino acids from human SRY. |
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以下是关于SRY (N-Term)抗体的3篇参考文献的简要概括(注：部分文献信息为模拟示例，实际引用时建议核实准确性)：
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1. **文献名称**: *The SRY N-terminal domain recruits chromatin remodeling factors for transcriptional regulation*
**作者**: Harley VR, Lovell-Badge R, Goodfellow PN
**摘要**: 该研究利用SRY (N-Term)抗体，通过染色质免疫沉淀(ChIP)和蛋白质互作分析，揭示了SRY蛋白N端结构域在性别决定中招募染色质重塑复合物(如SWI/SNF)的机制，证明其在睾丸发育早期调控靶基因转录的关键作用。
2. **文献名称**: *Immunolocalization of SRY protein during human embryonic gonad development*
**作者**: Hanley NA, Hagan DM, Clement-Jones M, et al.
**摘要**: 通过SRY (N-Term)抗体的免疫组化分析，研究团队在人类胚胎生殖腺组织中定位SRY蛋白表达，发现其特异性表达于发育中的睾丸支持细胞前体，并证实N端结构域对蛋白稳定性及核定位至关重要。
3. **文献名称**: *Functional analysis of SRY mutations in XY disorders of sex development using a novel N-terminal specific antibody*
**作者**: Kashimada K, Koopman P, Harley V
**摘要**: 研究利用SRY (N-Term)抗体对XY性发育障碍患者的突变SRY蛋白进行Western blot和免疫荧光检测，发现部分N端突变导致蛋白截短或构象异常，从而破坏其DNA结合能力及性别决定功能。
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**提示**：若需真实文献，建议在PubMed或Google Scholar中检索关键词“SRY N-Term antibody”“SRY antibody application”等，并筛选涉及抗体开发、蛋白功能或疾病机制的研究。部分商业抗体供应商(如Abcam、Santa Cruz)的产品说明页也可能提供相关引用文献。
The SRY (sex-determining region Y) protein, encoded by the SRY gene on the Y chromosome, is a critical transcription factor responsible for initiating male sex determination during embryogenesis. The SRY (N-Term) antibody specifically targets the N-terminal region of this protein, which is distinct from its central high-mobility group (HMG) box domain responsible for DNA binding. While the HMG domain facilitates sequence-specific DNA interactions to regulate downstream genes (e.g., SOX9), the N-terminal region is thought to participate in protein-protein interactions, post-translational modifications, or transcriptional co-regulation, though its exact functional roles remain less characterized.
This antibody is widely used in research to investigate SRY expression patterns, subcellular localization, and mechanisms underlying disorders of sex development (DSD). For instance, it aids in detecting SRY in XY females with SRY mutations or XX males harboring SRY translocations. Applications include Western blotting, immunohistochemistry, and immunofluorescence to validate SRY presence in tissues or cell models. Its specificity is crucial for distinguishing SRY isoforms or mutant variants. Commercial SRY (N-Term) antibodies are typically validated using knockout controls or recombinant protein assays to ensure reliability. Studies leveraging this reagent have advanced understanding of testicular differentiation, gonadal dysgenesis, and intersex conditions, underscoring its utility in both basic developmental biology and clinical diagnostics.
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