| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIM homeobox transcription factor 1-alpha, LIM/homeobox protein 11, LMX-11, LIM/homeobox protein LMX1A, LMX1A |
| Entrez GeneID | 4009 |
| WB Predicted band size | 42.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This LMX1A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 303-337 amino acids from the C-terminal region of human LMX1A. |
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以下是关于LMX1A抗体的3篇示例文献(虚构示例，供参考)：
1. **文献名称**：《LMX1A在胰腺癌中的表观遗传调控及临床意义》
**作者**：Smith J, et al.
**摘要**：本研究利用LMX1A特异性抗体，通过免疫组化分析发现LMX1A在胰腺癌组织中表达显著下调，其启动子甲基化与患者预后不良相关，提示LMX1A可能作为抑癌基因参与肿瘤进展。
2. **文献名称**：《LMX1A在中脑多巴胺神经元发育中的功能研究》
**作者**：Wang L, et al.
**摘要**：通过LMX1A抗体进行胚胎小鼠脑组织免疫荧光染色，证实LMX1A对中脑多巴胺神经元的定向分化至关重要，敲除LMX1A导致神经元标志物TH表达缺失。
3. **文献名称**：《LMX1A抗体在肾纤维化模型中的表达验证》
**作者**：Garcia R, et al.
**摘要**：采用Western Blot和免疫沉淀技术，验证了LMX1A抗体在肾纤维化小鼠模型中的特异性，发现LMX1A通过调控TGF-β通路促进纤维化进程。
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注：以上文献为模拟内容，实际研究中请通过PubMed或学术数据库检索具体文献。
The LMX1A antibody is a crucial tool in developmental biology and neuroscience research, targeting the LIM homeobox transcription factor 1 alpha (LMX1A). This protein plays a pivotal role in embryonic development, particularly in the dorsoventral patterning of the central nervous system and the specification of dopaminergic neurons in the midbrain. LMX1A is essential for regulating genes involved in neuronal differentiation, including *FOXA2* and *Nurr1*, and is critical for the development of midbrain dopamine neurons, which are implicated in Parkinson’s disease.
Researchers use LMX1A antibodies to study its expression patterns, localization, and functional roles in vitro and in vivo. These antibodies are commonly applied in techniques like immunohistochemistry, Western blotting, and immunofluorescence to identify LMX1A-expressing cells in tissues such as the embryonic midbrain, spinal cord, and retina. They help elucidate mechanisms underlying neurodevelopmental disorders, neurodegenerative diseases, and potential regenerative therapies.
LMX1A antibodies are also valuable in characterizing disease models, such as Parkinson’s, where dopaminergic neuron loss occurs. Specificity validation via knockout controls or siRNA knockdown is recommended, as cross-reactivity with related family members (e.g., LMX1B) may occur. Commercial LMX1A antibodies are typically raised against epitopes within its N-terminal LIM domains or C-terminal homeodomain, with molecular weights around ~37-40 kDa. Ongoing research continues to explore LMX1A’s broader roles in organogenesis and its potential as a biomarker or therapeutic target.
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