Mouse Monoclonal XIAP Antibody

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     All lanes : Anti-XIAP Antibody at 1:1000-1:4000 dilution Lane 1: Jurkat whole cell lysates Lane 2: Raji whole cell lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling XIAP with P33213 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG   secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     P33213 staining XIAP in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

XIAP (X-linked inhibitor of apoptosis protein), a member of the inhibitor of apoptosis (IAP) family, plays a critical role in regulating programmed cell death (apoptosis) and cellular survival. Structurally, it contains three baculovirus IAP repeat (BIR) domains that enable interactions with caspases, key enzymes in apoptosis. Specifically, XIAP inhibits caspase-3. -7. and -9 via its BIR2 and BIR3 domains, thereby blocking downstream apoptotic signaling. Beyond apoptosis, XIAP is involved in inflammatory pathways, immune regulation, and cellular signaling, including NF-κB activation.

Dysregulation of XIAP is linked to diseases such as cancer (overexpression promotes therapy resistance), neurodegenerative disorders, and X-linked lymphoproliferative syndrome type 2 (XLP-2), caused by XIAP mutations. XIAP antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry. Therapeutically, XIAP-targeting antibodies or small-molecule inhibitors are explored to sensitize cancer cells to apoptosis or modulate immune responses. Monoclonal and polyclonal XIAP antibodies vary in specificity, enabling diverse research applications. Overall, XIAP antibodies bridge fundamental research and clinical translation in diseases tied to apoptosis dysregulation.