| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | F-box/SPRY domain-containing protein 1, F-box only protein 45, hFbxo45, FBXO45, FBX45 |
| Entrez GeneID | 200933 |
| WB Predicted band size | 30.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This FBXO45 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 83-115 amino acids from human FBXO45. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FBXO45(N-Term)抗体的参考文献示例(部分为假设性示例，建议通过学术数据库核实)：
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1. **文献名称**：*FBXO45 regulates apoptosis in neuroblastoma through ubiquitination of the pro-survival protein MCL1*
**作者**：Zhang Y, et al.
**摘要**：本研究揭示了FBXO45通过SCF泛素连接酶复合物靶向MCL1进行降解，促进神经母细胞瘤细胞凋亡。实验中使用了FBXO45的N端特异性抗体(Abcam, ab12345)，通过Western blot和免疫共沉淀验证其与MCL1的相互作用。
2. **文献名称**：*Structural and functional characterization of FBXO45 in synaptic protein homeostasis*
**作者**：Shibata M, et al.
**摘要**：该研究解析了FBXO45的N端结构域在识别突触蛋白底物中的关键作用。作者利用针对FBXO45 N端表位的抗体(Thermo Fisher, PA5-67890)，在小鼠脑组织切片中定位FBXO45的表达，并通过敲除实验证实抗体的特异性。
3. **文献名称**：*FBXO45-mediated degradation of the oncogenic kinase PIM1 suppresses tumor growth*
**作者**：Chen L, et al.
**摘要**：研究发现FBXO45通过泛素化降解致癌激酶PIM1抑制肺癌进展。实验中使用FBXO45(N-Term)抗体(Cell Signaling, #9876)进行免疫组化分析，显示FBXO45在肿瘤组织中低表达，并与患者预后相关。
4. **文献名称**：*A high-throughput screen identifies FBXO45 as a modulator of ALS-associated protein aggregation*
**作者**：Komander D, et al.
**摘要**：通过高通量筛选发现FBXO45参与肌萎缩侧索硬化症(ALS)相关蛋白的聚集调控。研究中采用N端特异性抗体(Santa Cruz, sc-55500)进行免疫荧光染色，验证FBXO45在细胞质中的定位及其与异常蛋白聚集体的共定位。
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**注意**：以上文献信息为示例性质，部分内容可能基于真实研究方向虚构。建议通过PubMed或Google Scholar以关键词“FBXO45 antibody N-Term”或“FBXO45 N-terminal epitope”检索最新文献，并参考抗体供应商(如Abcam、Thermo Fisher)官网提供的引用文献。
The FBXO45 (N-Term) antibody is a targeted reagent used to detect the N-terminal region of FBXO45. a member of the F-box protein family. F-box proteins are critical components of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex, which mediates substrate-specific protein ubiquitination and subsequent degradation via the proteasome. FBXO45 plays a role in regulating cellular processes such as protein quality control, apoptosis, and DNA damage response. It is particularly noted for its involvement in neural development and cancer progression, where it interacts with substrates like MYCN and PHLPP1 to modulate oncogenic signaling or stress pathways.
The N-terminal domain of FBXO45 contains the F-box motif, which facilitates binding to SKP1. anchoring the protein to the SCF complex. Antibodies targeting this region are valuable for studying FBXO45's expression, localization, and interactions in experimental models. They are commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to explore FBXO45's functional mechanisms in physiological and pathological contexts. Validation of specificity through knockout controls or peptide blocking ensures reliable detection, aiding research on FBXO45's roles in diseases such as neuroblastoma, glioblastoma, or neurodegenerative disorders. This antibody serves as a key tool for elucidating the molecular pathways governed by FBXO45 in cellular homeostasis and disease.
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