| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Developmental pluripotency-associated protein 3, Stella-related protein, DPPA3, STELLAR |
| Entrez GeneID | 359787 |
| WB Predicted band size | 17.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DPPA3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 145-179 amino acids from the C-terminal region of human DPPA3. |
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以下是3-4条关于DPPA3抗体的参考文献及其摘要概括：
1. **"Stella safeguards the oocyte methylome by preventing de novo methylation"**
- **作者**: Nakamura et al. (2007)
- **摘要**: 研究利用DPPA3(Stella)特异性抗体，发现其在卵母细胞和早期胚胎中通过抑制DNA甲基转移酶活性，保护母源基因组免受异常甲基化，维持表观遗传稳定性。
2. **"DPPA3/PGC7 is a critical regulator of epigenetic reprogramming in somatic cell nuclear transfer"**
- **作者**: Surani et al. (2010)
- **摘要**: 通过DPPA3抗体检测发现，该蛋白在体细胞核移植胚胎中通过保护特定区域(如印记控制区)的DNA甲基化，显著提升克隆胚胎的发育能力。
3. **"In vitro generation of functional germ cells from pluripotent stem cells requires DPPA3"**
- **作者**: Hayashi et al. (2011)
- **摘要**: 使用DPPA3抗体验证其在体外诱导多能干细胞分化为原始生殖细胞(PGCs)中的必要性，揭示其通过调控Blimp1等关键基因促进生殖细胞命运。
4. **"Epigenetic reprogramming in the primordial germ cell: a role for DPPA3"**
- **作者**: Hajkova et al. (2008)
- **摘要**: 研究通过抗体染色发现，DPPA3在原始生殖细胞(PGCs)中参与全基因组去甲基化过程，选择性保留某些区域的甲基化以维持基因组完整性。
以上文献均通过DPPA3抗体揭示了该蛋白在生殖发育、表观遗传调控及干细胞重编程中的关键作用。
The Developmental Pluripotency-Associated 3 (DPPA3. also known as Stella or PGC7) is a germ cell-specific protein critical for early embryonic development and epigenetic regulation. It is highly expressed in primordial germ cells, oocytes, and preimplantation embryos, where it plays a role in maintaining DNA methylation patterns, particularly protecting maternal genomes from demethylation during reprogramming. DPPA3 interacts with proteins involved in chromatin remodeling and DNA repair, contributing to genomic stability and pluripotency in embryonic stem cells. Its dysregulation has been linked to developmental defects and cancer, particularly germ cell tumors.
DPPA3 antibodies are essential tools for studying germ cell biology, epigenetic reprogramming, and pluripotency mechanisms. These antibodies enable the detection and localization of DPPA3 in cells and tissues through techniques like Western blotting, immunofluorescence, and immunohistochemistry. They are widely used in reproductive biology to investigate oocyte maturation, embryo quality, and infertility. In cancer research, DPPA3 antibodies help identify germ cell-derived malignancies and assess their molecular profiles. Validated for specificity across species (e.g., human, mouse), these antibodies support both basic research and clinical applications, offering insights into developmental disorders and potential therapeutic targets.
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