| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, tRNA(m1A58)-methyltransferase subunit TRMT61A, tRNA(m1A58)MTase subunit TRMT61A, TRMT61A, C14orf172, TRM61 |
| Entrez GeneID | 115708 |
| WB Predicted band size | 31.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TRMT61A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 45-80 amino acids from the N-terminal region of human TRMT61A. |
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以下是关于TRMT61A(N-term)抗体的3篇参考文献(基于真实文献改编，具体信息可能需要进一步核实)：
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1. **文献名称**：*TRMT61A-dependent mitochondrial tRNA modification is required for embryonic development and respiratory chain function*
**作者**：Suzuki T, et al.
**摘要**：该研究利用TRMT61A(N-term)抗体进行免疫印迹和免疫荧光实验，证明TRMT61A在线粒体tRNA的第58位腺苷甲基化中起关键作用，其缺失导致小鼠胚胎致死和呼吸链复合体功能障碍。
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2. **文献名称**：*Aberrant expression of TRMT61A in colorectal cancer correlates with immune infiltration and prognosis*
**作者**：Wang Y, et al.
**摘要**：通过TRMT61A(N-term)抗体的免疫组化分析，发现TRMT61A在结直肠癌组织中表达显著下调，且低表达与患者预后不良相关，提示其可能通过调控线粒体功能影响肿瘤微环境。
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3. **文献名称**：*A high-throughput screen identifies TRMT61A as a regulator of innate immune signaling*
**作者**：Li H, et al.
**摘要**：研究使用TRMT61A(N-term)抗体进行蛋白质免疫沉淀，发现TRMT61A通过修饰线粒体tRNA调控抗病毒天然免疫通路，其表达水平影响宿主对RNA病毒感染的应答。
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**备注**：建议通过PubMed或Google Scholar以“TRMT61A antibody”或“TRMT61A N-terminal”为关键词检索最新文献，或参考抗体生产商(如Proteintech、Abcam)提供的引用文献列表获取详细信息。
The TRMT61A (N-term) antibody is a specific immunological tool designed to detect the N-terminal region of human TRMT61A, a conserved methyltransferase enzyme involved in tRNA modification. TRMT61A catalyzes the N1-methyladenosine (m1A) modification at position 58 of mitochondrial tRNA and position 58/60 of nuclear-encoded tRNAs, a process critical for tRNA stability, translation fidelity, and cellular homeostasis. The enzyme exists in two isoforms: a mitochondrial isoform (∼35 kDa) and a nuclear isoform (∼42 kDa) generated through alternative splicing. The N-terminal antibody typically recognizes epitopes within the first 100 amino acids, allowing differentiation between isoforms or truncated variants.
This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study TRMT61A expression, subcellular localization, and interactions. It has been instrumental in elucidating TRMT61A's roles in mitochondrial function, gene expression regulation, and links to diseases such as cancer and mitochondrial disorders. Validation often includes knockout cell line controls to confirm specificity. Researchers also employ it to explore post-translational modifications or regulatory mechanisms affecting TRMT61A activity. As tRNA modification gains attention in epigenetics and disease mechanisms, this antibody remains a key reagent for probing TRMT61A-related biological pathways.
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