| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rap guanine nucleotide exchange factor 1, CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G, RAPGEF1, GRF2 |
| Entrez GeneID | 2889 |
| WB Predicted band size | 120.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RAPGEF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 536-569 amino acids from the Central region of human RAPGEF1. |
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以下是关于RAPGEF1抗体的3篇参考文献示例(注：文献信息为模拟虚构，仅供参考格式)：
1. **文献名称**：*RAPGEF1 regulates endothelial barrier function through Rap1 signaling*
**作者**：Zhang Y, et al.
**摘要**：研究利用RAPGEF1抗体进行Western blot和免疫荧光实验，发现RAPGEF1通过激活Rap1信号通路维持血管内皮细胞屏障完整性，其表达下调导致通透性增加。
2. **文献名称**：*Role of RAPGEF1 in neuronal development and synaptic plasticity*
**作者**：Kuroda S, et al.
**摘要**：通过免疫组化及免疫共沉淀(使用RAPGEF1特异性抗体)，揭示了RAPGEF1在小鼠海马神经元突触形成中的关键作用，并证实其缺失影响长期增强(LTP)。
3. **文献名称**：*RAPGEF1 as a potential biomarker in colorectal cancer progression*
**作者**：Chen L, et al.
**摘要**：采用RAPGEF1抗体对临床组织样本进行免疫组化分析，发现RAPGEF1在结直肠癌转移灶中高表达，并与患者不良预后显著相关，提示其作为治疗靶点的潜力。
如需真实文献，建议在PubMed或Google Scholar以“RAPGEF1 antibody”及“C3G antibody”为关键词检索，并筛选涉及功能研究或抗体应用的论文。
The RAPGEF1 antibody is a crucial tool for studying the Rap guanine nucleotide exchange factor 1 (RAPGEF1), also known as C3G, a protein involved in regulating cellular processes such as adhesion, proliferation, and differentiation. RAPGEF1 activates Rap GTPases by facilitating the exchange of GDP for GTP, thereby modulating signaling pathways like MAPK and integrin signaling. This protein plays roles in diverse physiological and pathological contexts, including cancer progression, neuronal development, and immune responses.
Antibodies targeting RAPGEF1 are widely used in research to detect its expression, localization, and interactions in tissues or cell lines. They enable techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore RAPGEF1's function in diseases. For instance, RAPGEF1 dysregulation has been linked to cancers (e.g., colorectal, leukemia) and neurodevelopmental disorders, making its antibody valuable for mechanistic studies. These antibodies are often validated for specificity using knockout controls or siRNA knockdowns. Researchers also utilize them to investigate post-translational modifications or subcellular redistribution of RAPGEF1 under stimuli like growth factors or stress. Commercial RAPGEF1 antibodies are typically raised in hosts like rabbits or mice, with options for monoclonal or polyclonal formats, catering to diverse experimental needs.
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