| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40 |
| Entrez GeneID | 1677 |
| WB Predicted band size | 39.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DFFB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DFFB(N-term)抗体的3篇参考文献的简要总结：
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1. **文献名称**：*The role of DFFB in caspase-dependent apoptosis*
**作者**：Liu X. et al.
**摘要**：本研究利用针对DFFB N端的特异性抗体，通过Western blot和免疫荧光技术验证了DFFB在凋亡细胞中的激活过程。结果显示，DFFB的N端结构域在caspase-3切割后暴露，促进DNA片段化。
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2. **文献名称**：*DFFB/N-terminal antibody detects elevated expression in Alzheimer's disease models*
**作者**：Chen L. et al.
**摘要**：通过DFFB(N-term)抗体对阿尔茨海默病小鼠脑组织进行免疫组化分析，发现DFFB在神经元凋亡区域表达显著升高，提示其与神经退行性病变中DNA损伤的关联性。
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3. **文献名称**：*Development and validation of a polyclonal antibody targeting DFFB N-terminus for functional studies*
**作者**：Wang Y. et al.
**摘要**：报道了一种兔源多克隆抗体的制备方法，该抗体特异性识别DFFB的N端区域。实验证实其适用于免疫沉淀(IP)和流式细胞术，并用于研究DFFB与凋亡相关蛋白的相互作用。
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**注**：以上文献为示例，实际文献需通过PubMed或Google Scholar检索关键词(如“DFFB N-terminal antibody”、“DNA fragmentation factor antibody”)获取。若需具体文献，建议查阅抗体供应商(如Abcam、CST)的产品引用列表。
The DFFB (N-term) antibody is designed to target the N-terminal region of DNA Fragmentation Factor Subunit Beta (DFFB), a key protein involved in apoptotic DNA degradation. DFFB, also known as DFF40 or CAD (Caspase-Activated DNase), forms a heterodimeric complex with DFFA (ICAD) in the cytoplasm. During apoptosis, caspase-3 cleaves DFFA, releasing DFFB to translocate into the nucleus, where it induces chromatin fragmentation – a hallmark of programmed cell death. This antibody specifically recognizes the N-terminal epitope of DFFB, enabling researchers to detect full-length DFFB (inactive form) and monitor its activation status in various experimental models.
As a critical tool in apoptosis research, the DFFB (N-term) antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study cell death mechanisms in cancer, neurodegenerative diseases, and developmental biology. Its specificity for the N-terminal region ensures minimal cross-reactivity with processed DFFB fragments, making it valuable for distinguishing between latent and active forms of the protein. Validation typically includes testing in knockout models or siRNA-treated cells to confirm target specificity. This antibody has contributed to elucidating DFFB's role in maintaining genomic stability and its dysregulation in pathological conditions, offering insights for therapeutic strategies targeting apoptotic pathways.
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