Mouse Monoclonal Fer Antibody

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     All lanes : Anti-Fer Antibody at 1:4000 dilution Lane 1: NIH/3T3 whole cell lysates Lane 2: mouse testis lysates Lane 3: mouse liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Fer with P33033 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG   secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing NIH/3T3 cells stained with P33033 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33033,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

Fer (FPS/FES related) antibody targets the Fer protein, a non-receptor tyrosine kinase first identified in the 1980s. Belonging to the Fes/Fer kinase family, Fer is encoded by the *FER* gene and is involved in regulating cellular signaling pathways. Structurally, Fer contains an N-terminal F-BAR domain, which mediates membrane interactions, and a central SH2 domain that facilitates protein-protein interactions by binding phosphotyrosine residues.

Fer kinase plays roles in diverse cellular processes, including cell adhesion, proliferation, differentiation, and cytoskeletal reorganization. It is activated by growth factors, cytokines, and integrin-mediated signaling, often interacting with receptors like EGFR and PDGFR. Dysregulation of Fer has been implicated in cancer progression, particularly in promoting metastasis through enhanced cell motility and invasiveness. Elevated Fer expression correlates with poor prognosis in breast, ovarian, and prostate cancers.

Antibodies against Fer are essential tools for studying its expression, localization, and function. They enable techniques such as Western blotting, immunohistochemistry, and immunoprecipitation, aiding in both basic research and clinical diagnostics. Recent studies also explore Fer inhibitors as potential anticancer therapeutics. Despite its established roles, Fer's interplay with other kinases and context-dependent signaling mechanisms remain active research areas, highlighting its complexity in cellular regulation and disease.