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Rabbit Polyclonal (Mouse)Melk Antibody

  • 中文名: (Mouse) Melk抗体
  • 别    名: Maternal embryonic leucine zipper kinase, Protein kinase PK38, mPK38, Tyrosine-protein kinase MELK, Melk, Kiaa0175, Pk38
货号: IPDX33027
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/25 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

参考文献

以下是3篇与小鼠(Mouse)MELK(Maternal Embryonic Leucine Zipper Kinase)抗体相关的模拟参考文献示例,基于典型研究领域概括:

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1. **文献名称**:*MELK regulates embryonic stem cell self-renewal and pluripotency through phosphorylation of Oct4*

**作者**:Nakano, H., et al.

**摘要**:本研究利用小鼠胚胎干细胞模型,通过特异性MELK抗体进行免疫沉淀和Western blot分析,揭示了MELK通过磷酸化Oct4蛋白调控干细胞多能性维持的分子机制。研究证实MELK抗体在小鼠细胞中的高特异性,并发现MELK缺失导致干细胞分化异常。

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2. **文献名称**:*Development of a monoclonal antibody against mouse MELK for immunohistochemical analysis in brain tumors*

**作者**:Choi, Y.J., et al.

**摘要**:报道了一种针对小鼠MELK蛋白的单克隆抗体的开发与验证。通过免疫组化(IHC)和流式细胞术,该抗体成功应用于胶质母细胞瘤小鼠模型的组织样本检测,证明MELK在肿瘤细胞中高表达且与预后相关。

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3. **文献名称**:*MELK inhibition suppresses intestinal stem cell proliferation in a colitis-associated cancer model*

**作者**:Lin, S., et al.

**摘要**:研究使用特异性MELK抗体检测小鼠肠道干细胞中MELK的定位与表达水平,发现MELK通过调控Wnt/β-catenin通路促进干细胞增殖。抗体在肠类器官和小鼠结肠癌模型中的有效性验证了其作为潜在治疗靶点的价值。

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4. **文献名称**:*Comparative analysis of MELK antibody specificity across mouse and human tissues*

**作者**:Smith, R.P., et al.

**摘要**:系统评估了多种市售MELK抗体在小鼠组织中的交叉反应性,通过敲除小鼠模型验证了部分抗体的假阳性问题,提出优化实验条件以减少非特异性信号,为MELK功能研究提供抗体选择指南。

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注:以上文献为示例性概括,实际文献需通过PubMed或Google Scholar等数据库检索确认。建议以“MELK antibody mouse”、“MELK kinase murine model”等关键词搜索最新研究。

背景信息

The Maternal Embryonic Leucine zipper Kinase (Melk), a serine/threonine-protein kinase, plays roles in cell cycle regulation, proliferation, and apoptosis. Initially identified for its expression during embryogenesis, Melk is upregulated in various cancers and is associated with tumor progression, stem cell maintenance, and therapy resistance. Its overexpression in cancer stem cells and involvement in DNA damage response pathways make it a potential therapeutic target. Mouse Melk antibodies are immunological tools specifically designed to detect and study the murine ortholog of this kinase in experimental models. These antibodies, often monoclonal or polyclonal, are generated by immunizing hosts with Melk-derived antigens, enabling applications like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate Melk's function in murine development, cancer biology, and stem cell dynamics. Validated antibodies undergo rigorous specificity testing (e.g., knockout validation) to ensure reliability. Research using these reagents has advanced understanding of Melk's role in signaling pathways, offering insights for preclinical studies targeting Melk in oncology and regenerative medicine.

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