| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G-protein coupled receptor 56, Protein TM7XN1, GPR56 N-terminal fragment, GPR56 NT, GPR56(N), GPR56 extracellular subunit, GPR56 subunit alpha, GPR56 C-terminal fragment, GPR56 CT, GPR56(C), GPR56 seven-transmembrane subunit, GPR56 7TM, GPR56 subunit beta, GPR56, TM7LN4, TM7XN1 |
| Entrez GeneID | 9289 |
| WB Predicted band size | 77.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This antibody is generated from a mouse immunized with a recombinant protein. |
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以下是关于GPR56抗体的3篇参考文献，涵盖其在不同研究中的应用及发现：
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1. **"GPR56. a novel secretin-like human G-protein-coupled receptor gene"**
*作者：Shashidhar S.等 (2005)*
**摘要**：该研究首次克隆并表征了GPR56基因及其编码的蛋白，开发了特异性抗体用于检测其在多种组织中的表达。研究发现GPR56在免疫系统和神经系统细胞中高表达，提示其在细胞粘附和信号转导中的潜在作用。
2. **"GPR56 regulates VEGF production and angiogenesis during melanoma progression"**
*作者：Iguchi T.等 (2008)*
**摘要**：通过GPR56抗体进行免疫组化和Western blot分析，揭示了GPR56在黑色素瘤中通过调控VEGF分泌促进血管生成的作用。研究表明，GPR56表达下调与肿瘤转移增强相关，为靶向治疗提供了依据。
3. **"GPR56 is highly expressed in neural progenitor cells and affects cortical development"**
*作者：Luo R.等 (2011)*
**摘要**：利用GPR56抗体在小鼠模型中研究其皮层发育功能，发现GPR56缺失导致神经元迁移障碍和脑回结构异常。抗体染色显示其在放射状胶质细胞中高表达，强调其对神经发育的关键调控。
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这些文献展示了GPR56抗体在基因功能解析、肿瘤机制研究和神经发育领域的应用，均通过实验验证了抗体的特异性及研究价值。
GPR56. a member of the adhesion G protein-coupled receptor (GPCR) family, plays critical roles in diverse biological processes, including brain development, tumor suppression, and immune regulation. Its extracellular region mediates cell-cell and cell-matrix interactions, while the intracellular domain activates signaling pathways like RhoA and PKC. Dysregulation of GPR56 is linked to neurological disorders (e.g., bilateral frontoparietal polymicrogyria), cancer progression, and immune dysfunction.
GPR56 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques such as Western blotting, immunohistochemistry, and flow cytometry. Polyclonal antibodies often target extracellular epitopes for detecting surface GPR56. while monoclonal antibodies may focus on specific domains for functional studies. Challenges in antibody development arise from GPR56’s complex post-translational modifications, such as proteolytic cleavage and glycosylation, which can affect antigen accessibility. Additionally, species specificity and cross-reactivity with other GPCRs require rigorous validation.
Recent studies highlight therapeutic potential, with GPR56-targeting antibodies explored in cancer immunotherapy and neural repair. However, standardized protocols for antibody validation and clearer links between GPR56 isoforms and diseases remain gaps in the field. Continued refinement of GPR56 antibodies will advance both basic research and clinical applications.
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