| WB | 1/1000 | Mouse, Rat |
| IF | 咨询技术 | Mouse, Rat |
| IHC | 咨询技术 | Mouse, Rat |
| ICC | 技术咨询 | Mouse, Rat |
| FCM | 咨询技术 | Mouse, Rat |
| Elisa | 咨询技术 | Mouse, Rat |
| Aliases | DNA (cytosine-5)-methyltransferase 3-like, Dnmt3l |
| Entrez GeneID | 54427 |
| WB Predicted band size | 48.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse, Rat |
| Immunogen | This Mouse Dnmt3l antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 258-290 amino acids from the Central region of mouse Dnmt3l. |
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以下是3篇与Mouse Dnmt3l抗体相关的文献概览：
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1. **文献名称**: *Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice*
**作者**: Bourc'his, D., et al.
**摘要**: 该研究利用特异性Dnmt3l抗体，通过免疫沉淀和免疫荧光技术，揭示了Dnmt3l与Dnmt3a/b协同调控小鼠母源印记基因甲基化的机制，证实抗体在小鼠胚胎及生殖细胞中的有效性。
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2. **文献名称**: *Structure and function of the mouse DNA methyltransferase gene: Dnmt3L stimulates de novo methylation by Dnmt3a*
**作者**: Chedin, F., et al.
**摘要**: 通过抗体验证Dnmt3l在小鼠体细胞中的表达模式，发现其作为辅助因子增强Dnmt3a的甲基转移酶活性，Western blot和免疫共沉淀实验表明抗体可用于蛋白互作研究。
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3. **文献名称**: **Antibody-based analysis of Dnmt3l nuclear localization during gametogenesis**
**作者**: Kato, Y., et al.
**摘要**: 研究开发了高特异性鼠源Dnmt3l多克隆抗体，通过免疫组化证明Dnmt3l在配子发生过程中定位于细胞核，并与染色质重塑复合物共定位，为生殖细胞表观遗传研究提供工具。
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(注：若需更多文献，可基于抗体应用场景补充筛选，例如ChIP-seq或转基因模型中的抗体验证研究。)
The mouse Dnmt3l antibody is a tool used to study Dnmt3-like (Dnmt3l), a catalytically inactive member of the DNA methyltransferase 3 family that plays a critical role in establishing genomic DNA methylation patterns during gametogenesis and early embryogenesis. Unlike its enzymatically active paralogs Dnmt3a and Dnmt3b, Dnmt3l lacks conserved methyltransferase motifs but acts as a regulatory cofactor, enhancing the binding and processivity of Dnmt3a/3b to chromatin. It is essential for imprinting, silencing transposable elements, and ensuring proper germ cell development.
Antibodies targeting mouse Dnmt3l are widely used in epigenetic research to investigate its expression, localization, and interaction partners in germ cells, embryonic stem cells, and somatic tissues. They enable techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to dissect Dnmt3l's role in de novo DNA methylation and its association with histone modifications (e.g., H3K4me0). Studies using these antibodies have revealed Dnmt3l's stage-specific expression during spermatogenesis and oogenesis, as well as its dysfunction in reproductive disorders or developmental anomalies. Validation often includes knockout controls to confirm specificity, given the protein's structural similarity to other Dnmt3 family members. This antibody remains pivotal for exploring links between DNA methylation dynamics and diseases like infertility or cancer.
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