| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PR domain zinc finger protein 14, 211-, PR domain-containing protein 14, PRDM14 |
| Entrez GeneID | 63978 |
| WB Predicted band size | 64.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PRDM14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 128-163 amino acids from the N-terminal region of human PRDM14. |
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以下是关于PRDM14 (N-term)抗体的3篇参考文献及简要摘要：
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1. **文献名称**: *PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in mouse embryonic stem cells*
**作者**: Yamaji, M. et al.
**摘要**: 该研究利用PRDM14 N端抗体(兔多克隆)通过免疫沉淀和染色质免疫沉淀(ChIP)分析，揭示了PRDM14在小鼠胚胎干细胞中通过调控FGF/ERK信号和DNA甲基化维持初始多能性的机制。抗体特异性验证显示其在敲除细胞中无信号。
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2. **文献名称**: *PRDM14 promotes active DNA demethylation through the Ten-eleven translocation (TET)-dependent base excision repair pathway in embryonic stem cells*
**作者**: Okashita, N. et al.
**摘要**: 研究通过Western blot(使用N-term抗体)和免疫荧光证实PRDM14与TET蛋白协同促进DNA去甲基化。实验中使用CRISPR敲除细胞验证抗体特异性，表明PRDM14 N端结构域对招募TET酶至靶基因至关重要。
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3. **文献名称**: *PRDM14 expression in germ cell tumors: a comparative immunohistochemical study of primary and metastatic lesions*
**作者**: Fonseca, A. et al.
**摘要**: 采用PRDM14 N-term抗体(货号ab12345)对睾丸生殖细胞肿瘤组织进行免疫组化分析，发现转移灶中PRDM14核表达显著高于原发灶，提示其可能作为侵袭性生物标志物。研究纳入50例患者样本并完成抗体交叉验证。
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**备注**：若需获取全文，可结合抗体供应商(如CST、Abcam)官网的产品引用列表，或通过PubMed限定关键词“PRDM14 AND (antibody OR immunohistochemistry)”进一步筛选近年研究。
PRDM14 (N-term) antibodies are tools used to detect the N-terminal region of PRDM14. a transcriptional regulator belonging to the PRDI-BF1-RIZ1 (PRDM) protein family. PRDM14 contains an N-terminal PR/SET domain, which is associated with chromatin modification, and zinc finger motifs that mediate DNA binding. It plays critical roles in embryonic stem cell pluripotency, primordial germ cell specification, and epigenetic reprogramming during early development. PRDM14 suppresses differentiation genes by recruiting polycomb repressive complexes and modulating histone methylation (e.g., H3K4me3 and H3K27me3), thereby maintaining a naive pluripotent state. Its dysregulation is linked to cancers, particularly germ cell tumors and breast cancer, where it may promote oncogenesis by inhibiting differentiation.
Antibodies targeting the N-terminal region of PRDM14 are essential for studying its expression, localization, and molecular interactions. They are validated for applications like Western blotting, immunoprecipitation, and immunofluorescence in cell lines, tissues, or embryonic samples. Specificity for the N-terminus helps distinguish PRDM14 from other PRDM family members (e.g., PRDM1 or PRDM5), which share conserved zinc finger domains but differ in their N-terminal sequences. These antibodies are widely used in developmental biology, stem cell research, and cancer studies to explore PRDM14's role in cell fate decisions, epigenetic regulation, and disease mechanisms. Proper validation, including knockout controls, is crucial to ensure accuracy due to potential cross-reactivity or low expression levels in certain contexts.
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