Rabbit Polyclonal AlkalinePhosphatase(ALPI) Antibody

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     Western blot analysis of lysate from human placenta tissue lysate,  using ALPI Antibody (Center)(Cat.  #P32789).  P32789 was diluted at 1:1000.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.    

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     Western blot analysis of lysate from human placenta tissue,using ALPI Antibody (Center)(Cat. #P32789).P32789 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.    

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     Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with P32789 Alkaline Phosphatase (ALPI) (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.    

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     Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody(Center) (Cat#P32789).  P32789 was diluted at 1:100 dilution.  A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded M.small intestine section using JAK2 Antibody(C-term)(Cat#P32789). P32789 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.    

Alkaline Phosphatase (ALPI), also known as intestinal alkaline phosphatase, is a tissue-nonspecific isoenzyme primarily expressed in the intestinal epithelium. It plays a critical role in detoxifying bacterial lipopolysaccharides (LPS), regulating gut microbiota, and maintaining intestinal barrier integrity. ALPI is involved in phosphate metabolism, lipid absorption, and inflammation modulation, making it a key player in gut homeostasis and innate immunity.

Antibodies targeting ALPI are widely used in research to study its expression, localization, and function in health and disease. They are essential tools for detecting ALPI via techniques like immunohistochemistry (IHC), Western blot, and immunofluorescence (IF). These antibodies help investigate ALPI's role in conditions such as inflammatory bowel disease (IBD), sepsis, obesity, and colorectal cancer, where its activity is often dysregulated. Additionally, ALPI antibodies contribute to exploring therapeutic strategies, including enzyme replacement or modulation, to address gut barrier dysfunction or metabolic disorders. Commercially available ALPI antibodies are typically validated for specificity against human or model organism antigens, though cross-reactivity varies. Understanding ALPI's regulatory mechanisms and interactions with gut pathogens or commensals remains a focus, driving the continued development and application of ALPI-targeted reagents in biomedical research.