Rabbit Polyclonal ATP5J Antibody

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     Western blot analysis of lysate from rat heart tissue lysate, using ATP5J Antibody (Center)(Cat.  #P32785).  P32785 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.    

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     ATP5J Antibody (Center) (Cat. #P32785) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the ATP5J antibody detected the ATP5J protein (arrow).    

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     Fluorescent confocal image of U251 cell stained with ATP5J Antibody (Center)(Cat#P32785).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ATP5J immunoreactivity is localized to Mitochondria significantly.    

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     ATP5J Antibody (Center) (Cat. #P32785)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP5J Antibody (Center) for immunohistochemistry.  Clinical relevance has not been evaluated.    

**Background of ATP5J Antibody**

The ATP5J antibody is a research tool designed to detect ATP synthase subunit F6 (ATP5J), a critical component of the mitochondrial ATP synthase complex (Complex V). This enzyme, located in the inner mitochondrial membrane, catalyzes ATP production during oxidative phosphorylation by utilizing the proton gradient across the membrane. ATP5J, also known as ATP synthase membrane subunit F6. is part of the F0 subunit of Complex V and plays a role in stabilizing the complex and facilitating proton transport.

Antibodies targeting ATP5J are widely used in studies investigating mitochondrial function, energy metabolism, and diseases linked to mitochondrial dysfunction, such as neurodegenerative disorders, cancer, and metabolic syndromes. They enable researchers to assess protein expression levels, subcellular localization (e.g., mitochondrial vs. cytosolic distribution), and alterations under experimental conditions like hypoxia, nutrient stress, or genetic manipulation.

Validation of ATP5J antibodies typically includes Western blotting, immunofluorescence, and immunohistochemistry, often confirmed using knockout cell lines or tissues to ensure specificity. Commercial antibodies may vary in clonality (monoclonal/polyclonal), species reactivity, and conjugation formats (e.g., HRP, fluorescent tags). Proper controls, such as mitochondrial enrichment verification, are essential for accurate interpretation.

Overall, ATP5J antibodies are indispensable for dissecting mitochondrial biology and its implications in health and disease.