| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3, Centaurin-beta-5, Cnt-b5, ACAP3, CENTB5, KIAA1716 |
| Entrez GeneID | 116983 |
| WB Predicted band size | 92.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ACAP3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 759-792 amino acids from the C-terminal region of human ACAP3. |
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以下是关于ACAP3抗体的3篇示例参考文献(注：文献为假设性示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *ACAP3 regulates cell migration through Arf6-dependent pathway*
**作者**: Someya, T., et al. (2016)
**摘要**: 本研究利用特异性ACAP3抗体探究其在细胞迁移中的作用，发现ACAP3通过调控Arf6的GTPase活性影响囊泡运输和细胞极性，为肿瘤转移机制提供了新见解。
2. **文献名称**: *Neuronal development requires ACAP3-mediated endocytic trafficking*
**作者**: Li, X., et al. (2018)
**摘要**: 通过免疫荧光结合ACAP3抗体，作者揭示ACAP3在小脑颗粒神经元发育中的关键作用，证明其缺失导致突触形成障碍及轴突导向异常。
3. **文献名称**: *ACAP3 antibody-based profiling identifies its overexpression in aggressive breast cancer*
**作者**: Zhang, Y., et al. (2020)
**摘要**: 开发高灵敏度ACAP3单克隆抗体，用于乳腺癌组织检测，发现ACAP3高表达与肿瘤侵袭性及不良预后显著相关，提示其作为潜在生物标志物。
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**建议**：如需具体文献，可通过PubMed或Google Scholar搜索“ACAP3 antibody” + “ArfGAP”等关键词获取最新研究。部分研究可能侧重抗体开发(如Western Blot/IF验证)或功能研究(如细胞动力学、疾病模型)。
ACAP3 (ArfGAP with Coiled-coil, ANK repeat, and PH Domain 3) is a member of the ACAP family of GTPase-activating proteins (GAPs) that regulate ADP-ribosylation factor (Arf) proteins, key regulators of membrane trafficking and cytoskeletal dynamics. Structurally, ACAP3 contains an N-terminal ArfGAP domain responsible for accelerating GTP hydrolysis in Arf GTPases, a central coiled-coil motif for oligomerization or protein interactions, ANK repeats for substrate recognition, and a C-terminal pleckstrin homology (PH) domain likely involved in membrane localization.
Functionally, ACAP3 is implicated in clathrin-mediated endocytosis and intracellular vesicle transport, particularly in neuronal and immune cells. Studies suggest its role in neurite outgrowth, synaptic vesicle recycling, and immune cell migration by modulating Arf6 activity. Dysregulation of ACAP3 has been linked to neurodevelopmental disorders and cancer metastasis, though its precise pathological mechanisms remain under investigation.
ACAP3-specific antibodies are critical tools for detecting protein expression, subcellular localization, and interaction partners via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Commercial antibodies are typically validated for specificity against recombinant ACAP3 or knockout cell lines. Recent research focuses on its crosstalk with signaling pathways like PI3K/AKT and potential as a therapeutic target, though functional studies remain limited compared to other ACAP family members (ACAP1/2).
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