| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sperm protein associated with the nucleus on the X chromosome A, Cancer/testis antigen 111, CT111, Nuclear-associated protein SPAN-Xa, SPAN-X, SPANX-A, SPANX family member A, SPANXA1, SPANXA |
| Entrez GeneID | 30014;728712 |
| WB Predicted band size | 11.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SPANXA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human SPANXA1. |
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以下是关于SPANXA1 (N-term)抗体的3篇参考文献的简要整理：
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### 1. **文献名称**
*"SPANXA1 is a novel cancer-testis antigen in hepatocellular carcinoma"*
**作者**: Li Y, et al.
**摘要**:
研究利用SPANXA1 (N-term)抗体进行免疫组化分析，发现SPANXA1在肝癌组织中异常高表达，且与肿瘤分期和预后相关。抗体特异性验证显示其对N端表位的高亲和力，提示其作为肝癌生物标志物的潜力。
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### 2. **文献名称**
*"Expression and functional characterization of SPANX proteins in human spermatozoa"*
**作者**: Kouprina N, et al.
**摘要**:
通过SPANXA1 (N-term)抗体的免疫荧光定位，证实SPANXA1蛋白在精子头部顶体区域富集，可能参与精子成熟和受精过程。研究强调了该抗体在生殖细胞研究中的特异性应用。
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### 3. **文献名称**
*"SPANX family members as potential therapeutic targets in ovarian cancer"*
**作者**: Grizzi F, et al.
**摘要**:
研究采用SPANXA1 (N-term)抗体进行Western blot和流式细胞术分析，发现卵巢癌细胞系中SPANXA1的表达与化疗耐药性相关，提示其可能成为卵巢癌治疗的靶点。
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**注**：以上文献信息为示例性内容，实际文献需通过PubMed、Web of Science等数据库检索确认。若需具体文献，建议结合抗体生产商(如Abcam、Sigma-Aldrich)提供的引用文献或相关领域综述进一步筛选。
The SPANXA1 (N-term) antibody is designed to detect the N-terminal region of the SPANXA1 protein, a member of the SPANX (sperm protein associated with the nucleus on the X chromosome) family. SPANXA1 is primarily expressed in the testis, particularly in spermatogenic cells, and is implicated in spermatogenesis and male fertility. It localizes to the nucleus and may play a role in chromatin organization, transcriptional regulation, or nuclear remodeling during sperm maturation. Aberrant expression of SPANXA1 has also been observed in certain cancers, including melanoma and prostate cancer, suggesting potential roles in tumorigenesis or cancer progression.
The SPANXA1 (N-term) antibody is commonly used in research applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to study the protein's expression, localization, and function in both normal and pathological contexts. Its specificity for the N-terminal region ensures recognition of full-length or truncated variants, depending on epitope availability. Validation often includes testing in cell lines or tissues with confirmed SPANXA1 expression and knockout controls to confirm signal absence in SPANXA1-deficient samples.
As SPANXA1 shares homology with other SPANX family members (e.g., SPANXB, SPANXC), careful validation is required to ensure minimal cross-reactivity. Researchers utilize this antibody to explore reproductive biology, cancer mechanisms, and potential diagnostic or therapeutic targets. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary by product, influencing experimental design and multiplexing compatibility.
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