| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALNT3 |
| Uniprot No | Q14435 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-633aa |
| 氨基酸序列 | QREVSVQYSKEESRMERNMKNKNKMLDLMLEAVNNIKDAMPKMQIGAPVR QNIDAGERPCLQGYYTAAELKPVLDRPPQDSNAPGASGKAFKTTNLSVEE QKEKERGEAKHCFNAFASDRISLHRDLGPDTRPPECIEQKFKRCPPLPTT SVIIVFHNEAWSTLLRTVHSVLYSSPAILLKEIILVDDASVDEYLHDKLD EYVKQFSIVKIVRQRERKGLITARLLGATVATAETLTFLDAHCECFYGWL EPLLARIAENYTAVVSPDIASIDLNTFEFNKPSPYGSNHNRGNFDWSLSF GWESLPDHEKQRRKDETYPIKTPTFAGGLFSISKEYFEYIGSYDEEMEIW GGENIEMSFRVWQCGGQLEIMPCSVVGHVFRSKSPHSFPKGTQVIARNQV RLAEVWMDEYKEIFYRRNTDAAKIVKQKAFGDLSKRFEIKHRLQCKNFTW YLNNIYPEVYVPDLNPVISGYIKSVGQPLCLDVGENNQGGKPLIMYTCHG LGGNQYFEYSAQHEIRHNIQKELCLHAAQGLVQLKACTYKGHKTVVTGEQ IWEIQKDQLLYNPFLKMCLSANGEHPSLVSCNPSDPLQKWILSQNDVDHH HHHH |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Mutations in GALNT3. encoding a protein involved in O-linked glycosylation, cause familial tumoral calcinosis"**
- **Authors**: Topaz O, et al.
- **摘要**: 该研究发现了GALNT3基因突变导致家族性肿瘤样钙质沉着症，表明GALNT3在磷酸盐代谢和钙化抑制中的关键作用。重组蛋白功能分析显示突变导致酶活性丧失，影响O-糖基化过程。
2. **"Recombinant expression and characterization of human GALNT3 in mammalian cells"**
- **Authors**: Ichikawa S, et al.
- **摘要**: 研究通过哺乳动物细胞系统成功表达并纯化重组人源GALNT3蛋白，证实其在体外催化黏蛋白类底物的O-糖基化活性，为疾病模型研究提供工具。
3. **"Structural insights into the catalytic mechanism of GALNT3 through recombinant protein crystallography"**
- **Authors**: Tran DT, et al.
- **摘要**: 利用重组GALNT3蛋白解析其晶体结构，揭示了底物结合域和催化机制，阐明糖基转移过程中关键氨基酸残基的功能。
4. **"Functional consequences of GALNT3 mutations on FGF23 glycosylation and metabolism"**
- **Authors**: Garringer HJ, et al.
- **摘要**: 研究通过重组GALNT3蛋白实验，证明突变导致FGF23蛋白异常糖基化，进而引发高磷血症，揭示了GALNT3在激素调控中的生理意义。
(注：上述文献信息为示例性质，实际引用需核实具体文献内容。)
**Background of GALNT3 Recombinant Protein**
The GALNT3 (polypeptide N-acetylgalactosaminyltransferase 3) recombinant protein is a key enzyme involved in *O*-glycosylation, a post-translational modification critical for protein stability, trafficking, and function. GALNT3 belongs to the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family, which initiates mucin-type *O*-glycosylation by transferring N-acetylgalactosamine (GalNAc) to serine or threonine residues on target proteins. This modification influences cell-cell communication, signaling, and tissue homeostasis.
GALNT3 is notably expressed in tissues like the kidney, liver, and endocrine glands. Its dysfunction is linked to human diseases, including familial tumoral calcinosis (FTC), a rare metabolic disorder characterized by hyperphosphatemia and ectopic calcification due to mutations in the *GALNT3* gene. GALNT3-mediated *O*-glycosylation regulates fibroblast growth factor 23 (FGF23), a hormone critical for phosphate metabolism. Impaired GALNT3 activity disrupts FGF23 processing, leading to metabolic imbalances.
Recombinant GALNT3 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), retains enzymatic activity and serves as a tool to study *O*-glycosylation mechanisms. It enables *in vitro* assays to identify substrates, characterize enzyme kinetics, and explore regulatory pathways. Additionally, recombinant GALNT3 aids in developing therapeutic strategies for FTC or related disorders by restoring FGF23 function.
In cancer research, GALNT3’s role is context-dependent, showing tumor-suppressive or pro-metastatic effects in different cancers. Recombinant GALNT3 helps dissect its dual functions in tumor progression and metastasis. Overall, GALNT3 recombinant protein is vital for advancing glycobiology research, disease modeling, and targeted drug development.
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