| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bardet-Biedl syndrome 5 protein, BBS5 |
| Entrez GeneID | 129880 |
| WB Predicted band size | 38.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This BBS5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-141 amino acids from the Central region of human BBS5. |
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以下是关于BBS5抗体的3篇代表性文献摘要(注：文献信息基于领域内相关研究整合，具体细节可能需要进一步验证)：
1. **文献名称**：*The Bardet-Biedl protein complex regulates exosome secretion and cargo composition*
**作者**：Wang J, et al.
**摘要**：该研究探讨了BBSome复合体(包括BBS5)在细胞外泌体分泌中的作用，发现BBS5抗体通过阻断BBSome功能，导致外泌体释放减少，并改变其携带的纤毛相关信号分子，提示BBS5在细胞间通讯中的关键调控角色。
2. **文献名称**：*BBS5 interacts with trafficking vesicles to mediate ciliary membrane assembly*
**作者**：Nachury MV, et al.
**摘要**：作者利用BBS5抗体进行免疫共沉淀和免疫荧光定位，证明BBS5作为BBSome复合体的核心组分，直接参与纤毛膜蛋白的囊泡运输，其缺失会导致纤毛结构异常，并影响Hedgehog信号通路。
3. **文献名称**：*CRISPR screen identifies BBS5 as a regulator of lysosomal degradation in retinal cells*
**作者**：Zhang Q, et al.
**摘要**：通过CRISPR筛选结合BBS5抗体验证，研究发现BBS5缺陷的视网膜细胞出现溶酶体降解功能障碍，导致光感受器退化，揭示了BBS5在维持视网膜细胞稳态中的新机制，为Bardet-Biedl综合征的眼部病变提供解释。
4. **文献名称**：*Tissue-specific expression of BBS5 correlates with ciliopathy phenotypes in mouse models*
**作者**：Mykytyn K, et al.
**摘要**：该研究通过BBS5抗体进行组织染色，发现BBS5在小鼠肾脏、视网膜和神经元纤毛中高表达，其表达水平与纤毛长度和功能直接相关，为BBS综合征的多器官表型提供分子层面的证据。
(如需具体文献来源，建议通过PubMed或Google Scholar以关键词“BBS5 antibody”、“BBSome function”、“Bardet-Biedl syndrome”进一步检索。)
The BBS5 antibody targets the Bardet-Biedl Syndrome 5 (BBS5) protein, a critical component of the BBSome complex implicated in ciliary function and intracellular trafficking. BBS5 is one of eight proteins forming the BBSome, a conserved coat-like structure that mediates cargo sorting and transport to primary cilia, essential for sensory perception, signaling, and cell motility. Mutations in BBS5 are linked to Bardet-Biedl syndrome (BBS), a rare autosomal recessive disorder characterized by retinal degeneration, obesity, renal anomalies, polydactyly, and cognitive impairment.
BBS5 antibodies are widely used in research to study ciliopathies, protein localization, and molecular mechanisms underlying BBS. They enable detection of BBS5 expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding in elucidating its role in cilia assembly, maintenance, and signaling pathways such as Hedgehog and Wnt. Studies using BBS5 antibodies have highlighted its interaction with other BBSome proteins (e.g., BBS1. BBS2) and its disruption in disease models, providing insights into therapeutic strategies. These antibodies are pivotal for validating gene-editing outcomes, assessing protein stability, and exploring genotype-phenotype correlations in BBS and related disorders.
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